正己烷染毒对大鼠视网膜病理形态学和超微结构的影响  

Histopathology and ultrastructure changes of retina in rats exposed to n-hexane

作　　者：陈瑛[1] 徐锦堂[2] 唐福星[3] 刘阳[4] 程欣[5] 

机构地区：[1]广州市第十二人民医院眼科,510620 [2]暨南大学医学院眼科研究室 [3]暨南大学医学院电镜室 [4]广州市第十二人民医院外科,510620 [5]暨南大学医学院组胚教研室

出　　处：《中华劳动卫生职业病杂志》2009年第7期416-420,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广东省科技计划项目（2006B36006015）;广东省医学科研基金（A2006566）

摘　　要：目的研究正己烷染毒对大鼠视网膜组织的损伤。方法将32只雄性sD大鼠随机分成对照组和正己烷染毒组。对照组：8只，吸人空气；染毒组：24只，采取静式吸入染毒方式，正己烷浓度为35．2咖，，每组6只，分别染毒1、3、7、14d后处死大鼠。采用光学显微镜和透射电子显微镜观察正己烷对视网膜组织病理学和超微结构的影响。结果对照组大鼠视网膜组织结构层次清楚，染色均匀，细胞形态规整。染毒组大鼠正己烷染毒7d和14d时视网膜出现明显变性，并随染毒时间延长损伤加重。染毒14d，大鼠光感受器外节失去正常的膜盘结构，部分膜盘溶解；内节线粒体肿胀甚至消失。外核层细胞水肿，核内染色质浓缩。内核层水平细胞、双极细胞和无长突细胞出现明显变性。节细胞胞质水肿，细胞器明显减少。外丛状层、内丛状层和神经纤维层神经突起水肿，突触内微丝和小泡减少。结论正己烷可造成大鼠视网膜组织的明显损害，将导致眼底病的发生。Objective To investigate the injury in the retina of rats exposed to n-hexane. Methods Thirty-two SD male rats were randomly divided into control group and four n-hexane groups. The rats in the four n-hexane groups inhaled 35.2 g/m3 n-hexane statically for 1, 3, 7 and 14 days respectively （6 rats in every group）while 8 rats in the control group inhaled air. Histopathology and ultrastructure changes of the retina of rats were analyzed. Results Rats in control group had clear layers of retinal structure, stained evenly and with regular cell shape. Retinal degeneration was observed in the rats exposed to n-hexane for 7 d and 14 d, and aggravated by degrees with time exposed to n-hexane. In the rats exposed to n-hexane for 14 d, the outer segments of photoreceptor were arranged in a confusing order, and topically there appeared dissolution; in the inner segments, mitochondria were swollen or disappeared. Pyknotic chromatin and cytoplasmic edema were observed in the outer nuclear layer. There were degeneration of horizontal cells, bipolar cells and amaerine cells in the inner nuclear layer. Cytoplasmic edema and organelle dissolution were observed in ganglionic cells. In the neurofibrous layer, outer and inner plexiform layers, there was neuron cell tuber edema, and the microfilament and racuole of synapse decreased. Conclusion The histopathology and ultrastructure of retina are damaged in the rats exposed to n-hexane, thus leading to ocular fundus disease.

关 键 词：正己烷 视网膜 病理学 

分 类 号：R686[医药卫生—骨科学]