镍冶炼烟尘诱导哺乳动物细胞遗传物质损伤作用的研究  

Genetic damage of mammalian cells induced by nickel-refining fumes

作　　者：金艳涛[1] 吴永会[1] 于翠平[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,150081

出　　处：《中华劳动卫生职业病杂志》2009年第7期421-425,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30571549）;黑龙江省高等学校青年学术骨干支持计划项目（1152G020）

摘　　要：目的研究2种镍冶炼烟尘致细胞毒性和DNA损伤的作用及其差异。方法以某镍冶炼厂的镍冶炼炉前沉降尘和镍精炼车间沉降尘为受试物，用噻唑蓝（MTT）染色的方法检测（受试物浓度分别为100．00、50．00、25．00、12．50、6．25、0．00μg／ml）作用6、12、18、24、36、48h的细胞相对存活率；单细胞凝胶电泳技术检测（受试物浓度分别为100．0、50．0、25．0、12．5、0．0μg／ml）作用2、4、8h其致细胞的DNA损伤。结果随着染毒时间的延长和染毒剂量的增加，细胞存活率下降；2种冶炼烟尘引起的细胞拖尾率、尾矩和细胞尾部DNA含量增加。在浓度为100．00μg／ml的2种烟尘作用细胞48h后，细胞的存活率仅为24．5％和26．5％；镍冶炼炉前沉降尘的各浓度组在不同时间诱导的细胞拖尾率和尾部DNA含量均高于阴性对照组，差异有统计学意义（P〈0．05），镍精炼车间沉降尘除12．5μg／ml各作用时间组和50．0μg／ml作用2h组外的各实验组在不同时间诱导的细胞拖尾率和尾部DNA含量均高于阴性对照组，差异有统计学意义（P〈0．05）。结论镍冶炼烟尘可以降低细胞的相对存活率，并不同程度地致NIH／3T3细胞的DNA损伤。Objective To study the effects of two kinds of nickel-refining tumes on DNA damage of NIH/3T3 cell and the difference. Methods NIH/3T3 cells were treated by two kinds of nickel fumes collected from smelting furnace and refining workshop of a nickel-smeltery, and PBS taken the place of nickel-smelting fumes was used as negative control. Several hours later, the cytotoxicity of on NIH/3T3 cells was detected with MTT colorimetric assay, and the DNA damage was also measured by comet assay （single cell gel electrophoresis）. Result With the extension of exposure time and increasing of concentration, the living rate of NIH/3T3 cells was decreased; the tail rate, tail extent moment and tail DNA percent of NIH/3T3 cell induced by these two refining fumes were increased. After cells were treated with 100.00 μg/ml of nickel-smelting fume for 48 h, the living rate of NIH/3T3 cells was 24.5% and 26.5% respectively. The tail length of NIH/3T3 cell induced by these two refining fumes was not significant difference. Tail DNA percent of NIH/3T3 cell induced by smelting furnace fume was higher than negative control group （P〈0.05）. The tail rate, and tail DNA percent （except 12.5 μg/ml and 50.0 μg/ml treated 2 h group） of NIH/3T3 cell induced by refining wokshop fume was higher than negative control group （P〈0.05）. Conclusion Nickel-smelting fume could depress the survival rate of NIH/3T3 cells ,and induce different degree DNA damage of NIH/3T3 cell.

关 键 词：镍 细胞毒性试验 免疫 DNA损伤 

分 类 号：R686[医药卫生—骨科学]