HPLC法测定天元颗粒中天麻素的含量  

Determination of Gastrodin in Tianyuan Granula by HPLC

作　　者：冯瑛[1] 夏正燕[1] 徐强[1] 

机构地区：[1]浙江省中药研究所杭州310023

出　　处：《中药新药与临床药理》2009年第4期354-356,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的测定天元颗粒中天麻素的含量。方法样品采用甲醇回流提取,经中性氧化铝柱用80%乙醇洗脱收集;色谱柱:Diamonsil C18柱(250mm×4.6mm,5μm),流动相:乙腈-0.15%磷酸水溶液(1∶99),检测波长:220nm,流速:1mL/min,柱温:30℃。结果天麻素在0.376～2.632μg之间线性关系良好,r=0.999995(n=7),平均回收率99.09%,RSD为1.5%(n=6)。结论该法准确、重复性好,可用于天元颗粒制剂及天麻药材的含量测定及质量控制。Objective To establish a method for the determination of gastrodin in Tianyuan Granula. Methods The sample was refluxed with methanol, then eluted with 80 % ethanol through the alumina column（neutral）. The chromatographic conditions were as follows： Diamonsil C18 chromatographic column（250 mm×4.6 ram, 5 μm）with a mobile phase of acetonitrile -0. 15 % phosphoric acid （1： 99）, the detection wavelength being at 220 nm and the flow rate being 1.0 mL · min^-1. Results A linearity was obtained from 0. 376 μg to 2. 632 μg of gastrodin, and the correlation was good （r=0.9999 95, n=7）.The average recovery was 99.09 % and RSD=1.50 % （n=6）. Conclusion The method for determination of gastrodin in Tianyuan Granula is accurate, and with a good reproducibility.

关 键 词：天元颗粒 天麻素 HPLC 

分 类 号：R284.1[医药卫生—中药学]