石菖蒲的薄层鉴别及β-细辛醚的含量测定  

Identification of Rhizoma Acori Talarinowii and Determination of Its β-asarone Content

作　　者：黎奕明 眭道顺[2] 

机构地区：[1]惠州市顺泰药业有限公司,广东惠州516007 [2]广州中医药大学第一附属医院,广州510405

出　　处：《中药新药与临床药理》2009年第4期369-370,共2页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立石菖蒲药材鉴定方法及质量控制标准。方法以来源、薄层色谱鉴别石菖蒲药材,用HPLC法测定其有效成分β-细辛醚含量。结果薄层鉴别中,石菖蒲供试品在与对照品相对保留时间相同的位置上,显相同颜色的斑点;HPLC法测得β-细辛醚在0.1～0.6μg范围内线性关系良好,其回归方程为Y=37158.2X-589.5,r=0.9997,平均回收率为96.78%(RSD=0.95%)。结论该方法简便可行、重复性好,可用于石菖蒲的质量控制。Objective To establish a method for identification and quality control of Rhizoma Acori Talarinowii. Methods The medicinal herb of Rhizoma Acori Talarinowii was identified by its origin and TLC. High - performance, liquid chromatography （HPLC） was used to determine the content of β-asarone in Rhizoma Acori Talarinowii. Results The results of TLC showed that there were some spots with the same color in the position having the same relative retention time between the tested medicinal material and the control. The results of HPLC showed that a good linearity was in the range of 0. 1 -0.6 μg, the regression equation was Y=37158.2X-589.5（ r =0. 999 7）, and the average recovery was 96.78 % （RSD = 0.95 % ）. Conclusion The method is simple and reproducible, and can be used for the quality control of Rhizoma Acori Talarinowii.

关 键 词：石菖蒲 Β-细辛醚 薄层鉴别 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]