狂犬病毒新型快速荧光斑点抑制实验方法的建立  

A novel rapid fluorescence focus inhibition test for the detection of rabies virus antibody

作　　者：梁红茹[1] 刘晓慧[1] 陈晶[1] 孙招金[1] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院,广州510642

出　　处：《中国人兽共患病学报》2009年第7期619-622,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目(30871876);教育部长江学者和创新团队发展计划(IRT0723);广东省自然科学基金团队项目(5200638)

摘　　要：目的建立一种新型的快速荧光斑点抑制实验用于狂犬病毒抗体的检测。方法本研究以携带绿色荧光蛋白基因的嵌合狂犬病毒HEP-GFP株为基础毒株,建立了一种新型快速荧光斑点抑制实验(RFFIT-GFP);并对多份人、犬、猫的血清进行了检测,还将该检测结果与传统的RFFIT,ELISA相比较。结果与结论RFFIT-GFP和RFFIT,ELISA测定的结果基本相一致,特异性都比较高,而且RFFIT-GFP是一种比它们更为简便、经济的检测方法。The objective of the present study was to establish a novel rapid fluorescence focus inhibition test （RFFIT GFP）for the detection of rabies virus antibody, in which a chimeric rabies virus expressing green fluorescent protein （HEP GFP） was used as the basic virus strain in RFFIT GFP assay, and a few serum samples from human, dog and eat were detected by this new method . The optimal serum dilution, virus dosage and infection time were determined in 24 serum samples from human, dog and cat by using RFFIT GFP, RFFIT and ELISA assays. The result showed that these 3 methods gave a good consistency. But RFFIT GFP was found to be more convenient and economic for the detection of rabies virus antibody.

关 键 词：狂犬病毒 绿色荧光蛋白 快速荧光斑点抑制实验 

分 类 号：S852.65[农业科学—基础兽医学]