首次从国内鉴定到猪肠道杯状病毒及其分子流行病学调查  

The first identification and molecular epidemiology of PEC in China

作　　者：黄泽彬[1] 余兴龙[1] 李润成[1] 谢小雨[1] 尹德明[1] 颜运秋[1] 白霞[1] 刘忠华[1] 丁建[1] 汪镇南[1] 黎满香[1] 

机构地区：[1]湖南农业大学动物医学院,长沙410128

出　　处：《中国人兽共患病学报》2009年第7期623-626,共4页Chinese Journal of Zoonoses

基　　金：湖南省科技重大专项基金(2007FJ1003);湖南农业大学人才引进基金(04YJ03)

摘　　要：目的了解国内猪札如病毒（猪肠道杯状病毒成员）的存在和流行情况。方法从多个腹泻猪场共采集腹泻猪粪便189份，采用RT-PCR方法进行分子流行病学调查。结果检出猪札如病毒阳性病料24份，阳性率为12.70%（24/189）。测序后与国外已发表的毒株比较，表明了所测定流行毒株的序列与猪札如病毒代表株Cowden相近（同源性介于75.6%-88.3%之间），证实了其均为猪札如病毒。结论首次发现我国猪群中存在猪札如病毒。A total of 189 stool samples from swine with diarrhea, collected in various porcine farms in the central region of China were tested for porcine enteric caliciviruses （PEC） member porcine sapoviruses （SaV） by reverse transcription polymerase chain reaction （RT-PCR） amplification using primers designed to detect porcine SaV. Selected amplicons were sequenced to establish phylogenetic relationships with reference strains. Porcine SaV were detected in 12.70% （24/189） of the samples. Phylogenetic studies based on partial RNA polymerase gene sequences indicated that the field strains of viruses isolated in China were closely related （75.6 88.3% identity） to the porcine SaV Cowden reference strain. These results provide evidence that caliciviruses of the genus sapovirus circulate in piglets in China, but further studies are needed to clarify their importance as cause of diarrhea. This is the first report of PEC in China.

关 键 词：猪肠道杯状病毒 猪札如病毒 腹泻 猪 

分 类 号：R181.8[医药卫生—流行病学] R512.5[医药卫生—公共卫生与预防医学]