格列喹酮对胰岛β细胞株HIT-T15细胞的促胰岛素分泌作用  

Gliquidone-stimulated insulin secretion features are different from glibenclamide in HIT-T15 cells

作　　者：刘述益[1,2] 汤志梅[1] 张建梅[1] 邱平[1] 田浩明[1] 李小玉[3] 张国富[4] 李秀钧[1] 

机构地区：[1]四川大学华西医院内分泌科,成都610041 [2]成都市第一人民医院内分泌科 [3]四川大学华西口腔医学院组织与细胞工程实验室 [4]四川大学华西医院实验医学科,成都610041

出　　处：《中国糖尿病杂志》2009年第7期498-501,共4页Chinese Journal of Diabetes

摘　　要：目的通过观察不同葡萄糖浓度下格列喹酮(Glq)刺激HIT-T15细胞分泌胰岛素的作用,了解Glq对β细胞的刺激模式。方法 HIT-T15细胞孵育于5、10、15mmol/L葡萄糖浓度的培养基中,8小时后加入Glq、格列本脲(Glb)、格列齐特(Glc),分别在不同时间点收集培养液,测定药物刺激前后的胰岛素分泌水平。结果在三种糖浓度下,Glq刺激的胰岛素分泌高峰均在加药后10分钟,在30分钟时又有一稍低的第二峰,随后逐渐下降,至180分钟后分泌速率为基础值的1.1～1.2倍;Glb呈单相分泌峰,峰值出现在45分钟,随后分泌速率逐渐下降,至180分钟后仍有基础值的1.5倍;在10 mmol/L糖浓度组,Glq刺激的胰岛素浓度时间曲线下面积(AUC)更小。Glc的分泌曲线类似Glq。结论 Glq与Glb对β细胞的刺激作用模式不同,前者较后者胰岛素分泌达峰时间更快、作用时间更短,AUC更小。Objective To determine whether gliquidone has any different effect on the insulinsecretory profiles of HIT-T15 cells from gliclazide and glibenclamide in response to glucose stimuli. Methods HIT-T15 cells （β-cells line） were cultured for 8h in RPMI-1640 with three different glucose concentrations （5, 10, 15mmol/1）, the cells were re-cultured with gliquidone,gliclazide and glibenclamide in physiological concentrations, and then, insulin levels in the medium at 0,10,20,30,45,60,120 and 180 minutes were measured by radiommunoassay （RIA）. Results In this study, all three sulfonylureas strongly stimulated the insulin release in various concentrations in the present of glucose. Gliquidone and gliclazide induced a similarly rapid biphasic insulin secretion, reaching the first peak at 10 minutes and the second lower peak at 30 minutes, followed by gradual decline to near basal values at the 180 minutes. In contrast, glibenclamide induced a strong but monophasic insulin release, reaching its peak at 45 minutes followed by somewhat decline of the hormone release but did not return to basal values at 180 minutes. The undercurve area of insulin secretion was significantly smaller in gliquidone than in glibenclamide. Conclusions Gliquidone stimulates insulin release of HIT-T15 cells in biphasic manner which is similar to gliclazide, but different from glibenclamide displaying monophasic pattern. This unique feature in insulin secretion of gliquidone may have important implications in its clinical use.

关 键 词：胰岛素 HIT-T15细胞 格列喹酮 格列本脲 格列齐特 

分 类 号：R96[医药卫生—药理学]