香兰素衍生物VND3207对γ射线照射人淋巴母细胞基因组损伤和凋亡的保护作用  

PROTECTION OF VANILLIN DERIVATIVE VND3207 ON GENOME DAMAGE AND APOPTOSIS OF HUMAN LYMPHOBLASTOID CELLS INDUCED BY γ-RAY IRRADIATION

作　　者：黄锐[1,2] 贺性鹏[1] 徐勤枝[2] 王豫[2] 黄波[1,2] 周平坤[2] 

机构地区：[1]南华大学公共卫生学院,湖南省衡阳市421001 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《辐射防护》2009年第4期225-231,共7页Radiation Protection

基　　金：国家科技部重大专项课题(课题号:2008ZXJ09001-008);国家自然科学基金资助课题(课题号:3067634)

摘　　要：采用单细胞凝胶电泳、微核实验、AnnexinV标记结合流式细胞术、双荧光染色结合荧光显微镜观察等方法，分别检测不同浓度的VND3207对^60Coγ射线照射人淋巴母细胞AHH-1基因组损伤和凋亡的防护作用。中性单细胞凝胶电泳结果显示，5—40μmol／LVND3207能显著减轻2Gy^60Coγ射线照射AHH-1细胞的即刻DNA双链断裂损伤，其表现为与未加药组相比，VND3207保护组细胞的DNA双链断裂损伤（彗星尾矩）显著减小（p〈0．01），保护效果随着药物浓度增加更明显。同样，VND3207在5～40μmol／L浓度下，能显著降低0．5Gy、1．0Gy和2．0Gy^60Coγ射线照射AHH-1细胞的微核率，且呈现良好的剂量-效应关系，其中，40μmol／L浓度作用下2．0Gy照射细胞的微核率减少40％。Annexin V标记结合流式细胞术和双荧光染色法检测均显示，4．0Gy^60Coγ射线照射AHH-1细胞后8～48h，凋亡发生率随着时间延长而增加，40μmol／LVND3207能显著降低4．0Gy照射诱发的凋亡率及坏死率。本实验结果表明，VND3207对^60Coγ射线致细胞基因组损伤和细胞凋亡均有良好的保护作用。To determine the protective effect of vanillin derivative VND3207 on the genome damage and apoptosis of human lymphoblastoid AHH-1 cells induced by γ-ray irradiation, the techniques of single-cell gel electrophoresis, micronueleus test, Annexin V-FACS assay, and the double-fluorescein staining and fluorescent microscope observation were used. Neutral single-cell gel electrophoresis showed that the initial DNA double-strand breaks caused by 2 Gy ^60Co γ-ray was significantly decreased by VND3207 in the range of 5-40 μ mol/L. This significant phenomenon was demonstrated by the fact that the comet tail-moment was significantly shortened and the DNA content in the comet tail was reduced when the cells were protected with VND3207, and the radio-protective effect increases along with the increasing of drug concentration. Similarly, the yield of micronucleus was reduced by 5-40 μmol/L of VND3207 in a concentration-dependency in AHH-1 cells irradiated with 0.5 Gy, 1.0 Gy and 2.0 Gy ^60Co γ-rays. 40 μmol/L VND3207 resulted in 40% reduction in the yield of micronucleus induced by 2.0 Gy. The occurrence of apoptosis enhanced along with the time from 8 h to 48 h post-4 Gy irradiation, and 40 μ mol/L of VND3207 significantly decreased the induction of apoptosis. This work has further demonstrated a good protection of VND3207 on γ-ray-induced cell genome damage and apoptosis.

关 键 词：Γ射线 辐射防护 微核 DNA损伤 凋亡 香兰素衍生物 

分 类 号：R144[医药卫生—公共卫生与预防医学]