BEP2D细胞受照前后蛋白质组学研究  

PROTEOME ANALYSIS FOR EFFECTS OF RADIATION ON BEP2D CELL

作　　者：薛振伟[1] 周小林[1] 谷娟娟[1] 蘧艳峰[1] 崔梅萍[1] 

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所,太原030006

出　　处：《辐射防护》2009年第4期237-242,共6页Radiation Protection

基　　金：中国辐射防护研究院基础研究项目(项目编号:A0120060527-06)

摘　　要：本研究利用蛋白质组学技术对受1.5Gy、3Gy γ射线照射的人支气管上皮(BEP2D)细胞和未经照射的人支气管上皮细胞蛋白差异表达谱进行了分析与鉴定。研究结果表明:2-D电泳后在分子量14.4～94kD,等电点3～10范围内分离出约1000多个不同蛋白质斑点。凝胶图象显示1.5Gy的γ射线照射后与对照组相比有15个蛋白点上调,104个下调;3Gy的γ射线照射后有26个蛋白点上调,144个下调;二个不同照射剂量共同表达的差异蛋白点有18个,其中上调的有3个,下调的有15个。通过对选取的10个蛋白点进行质谱分析,有2个蛋白点(样品D和样品660)经检索鉴定为肌球蛋白轻链(MLC)。这些蛋白表达量的改变可能与辐射诱发肺部肿瘤的发生有关,为进一步研究肺癌的发病机理提供了一定的基础。The study analyzed the differential expression of proteins profile of the normal BEP2D cell and the BEP2D cell radiated with 1.5 Gy, 3 Gy γ rays by Proteomics technique. The results showed that approximately 1000 analyzed proteins per 2-D gel were isolated with molecular weights ranging between 14.4-94 kD and pI 3-10. Image analysis showed that irradiation with 1.5 Gy γ-rays contracting with control image caused down-regulation of 104 kinds of cell proteins and up-regulation of 15 proteins. Irradiation with 3 Gy γ-rays contracting with control image caused down-regulation of 144 kinds of cell proteins and up-regulation of 26 proteins. Irradiation with the two dimensional electrophoresis contracting with control image both caused down-regulation of 15 kinds of cell proteins and up-regulation of 3 proteins. Using mass spectrometry, a protein MLC was identified within the two protein spots （spot D and spot 660）. All of this change of proteins expressing may result in the lung cancer,and the study can provide baisis for the study of lung cancer pathogenesis.

关 键 词：蛋白质组 BEP2D细胞 肌球蛋白轻链 Γ射线 

分 类 号：Q274[生物学—细胞生物学]