PC-1增强受体酪氨酸激酶家族成员EphA3在前列腺癌细胞中的转录  

PC-1 Enhances Transcription of EphA3,a Member of RTK Family,in Prostate Cancer Cells

作　　者：武瑞琴[1] 李其满[1,2] 施庆国[1] 张惠[1] 钱晓龙[1] 俞岚[1] 周建光[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850 [2]福建农林大学,福州350002

出　　处：《中国生物化学与分子生物学报》2009年第7期633-639,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.30770834,30870961);国家高技术研究发展计划项目(863计划,No.2008AA02Z123)~~

摘　　要：为了研究前列腺癌相关基因(prostate and colon gene 1,PC-1)对受体酪氨酸激酶家族分子EphA3表达的影响,用RT-PCR、实时PCR和Western印迹检测表达不同水平PC-1的前列腺癌细胞系LNCaP和C4-2中EphA3的表达情况.发现PC-1可诱导EphA3基因表达上调.采用荧光素酶实验检测PC-1对于EphA3启动子转录活性的影响,结果显示,PC-1对转录起始位点上游916 bp的启动子活性没有影响,而可增强转录起始位点上游2011 bp启动子的活性.对EphA3启动子-916 bp～-2 011 bp区域进行生物信息学分析,结果显示,此区域包含HSF、NF-1、Nkx-2、SP1和GATA-1等多种转录因子结合位点.实验结果表明,PC-1可通过影响EphA3启动子诱导EphA3基因高表达,其调控区域位于转录起始位点上游-2 011 bp至-916 bp之间,提示PC-1可能通过影响一些结合于此区域的转录因子来影响EphA3启动子的转录活性.Expression of EphA3, which is a member of RTK family, was detected in prostate cancer cell lines LNCaP and C4-2 with different expression levels of prostate cancer associated gene PC-1 （prostate and colon gene 1）to investigate whether PC-1 influences the expression of EphA3. The results of RT-PCR, real-time PCR and Western blotting indicated that expression of EphA3 could be induced by PC-1. Results of luciferase assay showed that PC-1 had no influence on promoter of 916 bp upstream the transcriptional start site but can enhance the activity of promoter of 2 011 bp upstream the transcriptional start site. Many transcriptional factors such as HSF, NF-1, Nkx-2, SP1 and GATA-1 were predicted to bind to the region from - 916 bp to - 2 011 bp of EphA3 promoter. All the results showed that PC-1 can increase the expression of EphA3 by enhancing the activity of its promoter and the region from - 916 bp to - 2 011 bp of EphA3 promoter was involved in the regulation by PC-1, suggesting that PC-1 may regulate EphA3 promoter by influencing transcriptional factors binding to this region.

关 键 词：前列腺癌 前列腺癌相关基因(PC-1) 受体酪氨酸激酶 EphA3 

分 类 号：Q71[生物学—分子生物学]