实时荧光定量PCR检测布鲁菌方法的建立  

Detection of Brucella by Real-time PCR

作　　者：曹琛福[1] 刘学武 花群义[1] 吕建强[1] 秦智锋[1] 陈兵[1] 曾少灵[1] 孙洁[1] 

机构地区：[1]深圳出入境检验检疫局动植物检验检疫技术中心,广东深圳518010 [2]上海市崇明县动物疾病预防控制中心,上海崇明202100

出　　处：《动物医学进展》2009年第7期6-9,共4页Progress In Veterinary Medicine

基　　金：深圳出入境检验检疫局科技项目(SZ2007035)

摘　　要：旨在建立实时荧光定量PCR检测布鲁菌的方法。经选取高度保守的具布鲁菌属特异性的BSP31基因设计了一对引物及探针,并采用矩阵法优化反应体系,筛选出引物、探针、dNTP和Mg2+最优浓度配比,同时进行了特异性和灵敏性试验,建立了实时荧光定量PCR检测布鲁菌的方法。该方法可用于对布鲁菌病普查监测及进出境动物及其产品的检验检疫。This article was purposed of detection of Brucella spp by real-time PCR. A probe and a pair of primers were designed with BSP31 gene of Brucella, which is conversed domain in Brucella spp. The assay was optimized though matrix method, choseing the best concentration of the primers, probe, dNTP and Mg^2＋. The specificity and sensitivity of the assay were tested. This method can be used for general investigation of brucellosis in agriculture departments,and quarantine of entry-exit animals and their products.

关 键 词：布鲁菌 实时荧光定量PCR 检测 

分 类 号：S852.614[农业科学—基础兽医学]