免疫学与分子生物学技术在弓形虫病诊断中的研究进展  

Progress on Immunological and Molecular Biology Techniques for the Diagnosis of Toxoplasmosis

作　　者：孙秀涛[1] 谢银杰[1] 张思东[2] 沈学文[1] 赵阳 项勋[1] 段纲[1] 邹丰才[1] 

机构地区：[1]云南农业大学动物科技学院,云南昆明650201 [2]云南省动物疫病预防控制中心,云南昆明650201 [3]青岛市动物卫生监督所,山东青岛266001

出　　处：《动物医学进展》2009年第7期76-79,共4页Progress In Veterinary Medicine

基　　金：农业部公益性项目(200803017)

摘　　要：弓形虫病是一种呈世界性分布、危害严重的人兽共患寄生原虫病。弓形虫病给养殖业、公共卫生及经济发展带来严重危害。目前在国内还尚未有弓形虫相关疫苗。弓形虫感染的常用诊断方法包括临床诊断、病原学检查和血清学诊断等方法。但由于传统检测方法在特异性、敏感性和早期诊断方面存在一定的缺陷。因此,建立简便、快捷、敏感、特异的弓形虫病的检测方法对于检测弓形虫的感染显得尤为重要。论文主要从免疫学和分子生物学方面对该病的诊断和应用做一简要介绍。Toxoplasmosis is a serious and widespread parasitic zoonosis,which is harmful not only to animal husbandry,public health,but also to the development of economy. At present,the related vaccine of toxoplasmosis is not available in China. The diagnostic methods frequently used for detecting T. gondii infection in animals are clinic diagnosis, pathogenic diagnosis and serological tests which have limitations in specificity, sensitivity and early diagnosis. So it is especially important to develop more convenient,accurate, rapid, sensitive, specific and scientific diagnostic methods for the detection of the Toxoplasrna infections. The paper mainly reviewed the immunological and molecularbiology techniques for the diagnosis of toxoplasmosis.

关 键 词：弓形虫病 诊断技术 分子生物学 免疫学 

分 类 号：S854.43[农业科学—临床兽医学] S852.729[农业科学—兽医学]