解磷真菌C2'的分离鉴定及其在土壤中实际解磷效果的研究  

Phosphate-solubilizing Mechanism of C2' and Its Actual Phosphate-solubilizing Effect in Soil

作　　者：王莉晶[1] 高晓蓉[1] 吕军[1] 安利佳[1] 

机构地区：[1]大连理工大学环境与生命学院,辽宁大连116024

出　　处：《土壤通报》2009年第4期771-775,共5页Chinese Journal of Soil Science

基　　金：辽宁省基金资助项目(20062182)资助

摘　　要：以不溶性磷酸钙为筛选培养基,从墨西哥万年草根际土壤中分离筛选出一株高效解磷真菌C2'。经过形态观察和核糖体间隔区ITS扩增序列分析,确定该菌株为草酸青霉菌属(Penicillium oxalicum)。研究了不同碳源、氮源及盐浓度对其解磷能力的影响。利用高效液相色谱法(HPLC)对C2'的解磷机理进行初步研究,结果发现代谢过程中产生的苹果酸和酒石酸可能对溶磷起重要作用。土壤中实际的解磷效果表明,C2'对小麦苗期的生长有明显的促进作用。C2＇, capable of dissolving phosphorus, was isolated from mexican grass rhizosphere soil. It was identified as Penicillium oxalicum by the morphological observation and analysis of DNA sequences. The influence of different carbon sources, nitrogen sources and salt concentrations were carried out to find a best phosphate-solubilizing condition for C2＇. Malic and tartaric acids played a main role during the whole phosphate-solubilizing process through HPLC analysis. And phosphate-solubilizing microorganism C2＇ can make its promoting effects of wheat seedling growth, which may lay a foundation of the application of microbial fertilizers.

关 键 词：解磷真菌 解磷机理 解磷效果 

分 类 号：S144.1[农业科学—肥料学]