硫辛酸通过抑制NF-κB-iNOS-NO信号保护氧化应激诱导的PC12细胞损伤  

Lipoic Acid Protected PC12 Cells from Oxidative Stress-induced Damage by inhibiting the NF-κB-iNOS-NO Signal Pathway

作　　者：匡荣[1,2] 孙意国[1] 郑筱祥[1] 倪维芳[2] 朱社敏[2] 

机构地区：[1]浙江大学生物医学工程学院,教育部生物医学工程重点实验室,杭州310027 [2]浙江省食品药品检验所,杭州310004

出　　处：《中国现代应用药学》2009年第7期538-541,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的研究硫辛酸对氧化应激的PC12细胞NF-κB-iNOS-NO信号通路的影响。方法用终浓度为0.4mmol·L-1的H2O2损伤PC12细胞,用MTT法测定细胞存活率;用激光共聚焦法(LSCM)检测细胞内一氧化氮的动态变化;用RT-PCR法检测iNOS mRNA和NF-κBp65 mRNA表达量的变化。结果0.4mmol·L-1的H2O2处理PC12细胞4h,细胞存活率为27.9%(P<0.01),硫辛酸10mg·L-1可以提高H2O2损伤的PC12细胞存活率(65.4%,P<0.01);LSCM检测显示,DAF-2荧光强度的比值(Ft/F0)的最大值由损伤模型组的5.34下降到1.80;和模型组比较,iNOS mRNA和NF-κB p65 mRNA的表达明显下调(P<0.05或P<0.01)。结论硫辛酸可能通过抑制NF-κB-iNOS-NO信号减轻氧化应激诱导的PC12细胞损伤。OBJECTIVE To investigate the effect of lipoic acid（LA） on NF-κB-iNOS-NO signal pathway in oxidative stress-induced PC12 cells. METHODS After PC12 ceils were treated with 0.4 mmol·L^-1 H2O2 and different concentrations of LA, the cell viability was measured by MTT, the concentration of nitric oxide in intracellular cells was evaluated using laser-scanning confocal microscopy（LSCM） method and the expressions of NF-κB p65, iNOS mRNA were detected by RT-PCR. RESULTS Cell viability were significantly elevated by LA from 27.9% to 65.4%. Compared with H2O2-treated cells, the concentration of nitric oxide was lowed by LA through judging the change of DAF-2 F1/F0 from 5.34 to 1.80. Meanwhile, the levels of NF-κBp65 and iNOS mRNA were decreased （P〈0.05 or P〈0.01）. CONCLUSION Lipoic acid could protect PC12 cells from oxidative stress-induced damage by inhibiting the NF-κB-iNOS-NO signal pathway.

关 键 词：硫辛酸 过氧化氢 PC12细胞 一氧化氮 INOSMRNA NF-ΚB p65mRNA 

分 类 号：R977.22[医药卫生—药品]