我院常用抗癫痫药物人血药浓度的测定  

Blood Concentration Determination of Anti-epileptic Drugs in Our Hospital

作　　者：李峰[1] 张慧 

机构地区：[1]天津第一中心医院药剂科,天津300192 [2]天津天和医院药剂科,天津300050

出　　处：《中国现代应用药学》2009年第7期572-575,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的探讨建立苯巴比妥、苯妥英钠、卡马西平人血清浓度的测定方法,并应用该方法测定我院患者样本。方法采用高效液相色谱法(HPLC),流动相:乙晴-缓冲盐(30:70);柱温:37℃;检测波长:220nm;流速:0.7mL·min-1。含量测定分析采用内标对比法,杂质检查分析采用归一化法。结果该方法测定苯巴比妥、苯妥英钠、卡马西平的线性范围分别为4.58～75.88mg·L-1,2.25～35.95mg·L-1,1.26～20.08mg·L-1,日内和日间RSD均小于10%;回收率可控制在85%～115%之间。结论该方法具有良好的准确性、精密性和较高的灵敏度,且简便、快速、稳定。OBJECTIVE To establish a method to determine the concentration of Phenobarbital（PB）, phenytoin-sodium（PT） and carbamazepine（CBZ） in the human plasma, and determine the samples in our hospital with this method, METHODS The high performance liquid chromatography （HPLC） was used. The analysis conditions were as follows： acetonitrile-buffer salt solution （30 ： 70） as mobile phase, column oven temperature 37℃, wavelength 220 nm and flow rate 0.7 mL·min^-1. Internal standard method was developed to analyze the content of drugs, and normalization method was developed to analyze the content of impurity. RESULTS The linear ranges were 4.58-75.88 mg·L^-1, 2.25-35.95 mg·L^-1 and 1.26-20.08 mg·L^-1 for PB , PT and CBZ, respectively. The RSD within-day and between-days were both less than 10.0%. The average rate of recovery can be controlled in 85%-115%. CONCLUSION This method is simple, rapid, stable, with good accuracy, precision and high sensitivity.

关 键 词：高效液相色谱法 苯巴比妥 苯妥英钠 卡马西平 血药浓度监测 

分 类 号：R971.6[医药卫生—药品]