小麦回交后代中1 Dx 5+1 Dy 10优质亚基的分子检测  

Molecular Identification of High Quality Subunit 1 Dx 5+1 Dy 10 in Wheat Backcrossing Progenies

作　　者：任明见[1] 丁晓娟[2] 徐如宏[1] 王金洪 彭义 

机构地区：[1]贵州大学农学院,贵阳550025 [2]四川理工学院,四川自贡643000 [3]贵州省种子管理站,贵阳550001

出　　处：《种子》2009年第7期34-37,共4页Seed

基　　金：贵州省小麦"十一五"攻关项目(黔科合NY字[2006]3002);贵州省省长基金(20070208);贵州省农业动植物育种专项(黔农育专字[2007]03;[2008]02);贵州省科技成果重点推广计划项目资助(黔科合成字[2008]5022)

摘　　要：利用高分子量麦谷蛋白亚基1 Dx 5、1 Dy 10的PCR分子标记,对贵农21与加拿大小麦Neepawa的BC1 F2100个单株进行了分子标记辅助选择。结果表明,具有1 Dx 5和1 Dy 10亚基的亲本Neepawa和BC1F2代单株中可扩增出1Dx5(450bp)和1 Dy 10(576bp)特异带,具有12亚基的单株扩增出612bp特异带;在100个单株个体中,共检测到具有5+10优质亚基的5株,占5%;有2+12亚基的34株,占34%;同时具有5+10和2+12亚基的4株,占4%;并发现了新的组合类型5+12亚基,占57%。利用分子标记辅助选择技术,结合回交选育,可以在较早世代鉴定小麦优质亚基组合,选育出具有亚基组合新类型的小麦优质品系,为小麦的优质育种快速提供选育材料,从而提高选择效率,加快育种进程。This study used PCR molecular marker of high molecular glutenin 1 Dx 5 and 1 Dy 10 to carry on molecule marker-assisted selection for BC1 F1 of Guinong-21 and Neepawa wheat, total 100 plants were mark ered. Results showed that Neepawa with 1 Dx 5 and 1 Dy 10 and BC1F2 generation could amplify specific belt 1 Dx 5 with 450 bp and 1 Dy 10 with 57 6bp, and plant with 12 glutenin could amplify 612 bp specific belt. In 100 plants,5 plants with 5 ＋ 10 glutenin, 34 plants with 2 ＋ 12 glutenin, 4 plants with 5 ＋ 10 glutenin and 2 ＋ 12 glutenin. In this study , new type57 plants with 5 ＋ 12 glutenin were found. This study indicated that using molecular marker assisted selection technology, combining backeross breeding , could identify new assembles with high quality glutenin in early generation and breed new strain with high quality glutenin, and provide breeding materials quickly for wheat breeding with high quality , thus increasing selection efficiency and breeding procedure.

关 键 词：小麦 优质亚基 PCR MAS 

分 类 号：S512.1[农业科学—作物学]