柱前衍生化HPLC/UV法测定黄杨宁片中环维黄杨星D的含量  

Determination of cyclovirobuxine D in Huangyangning tablets by pre-column derivatization of HPLC/UV

作　　者：王大果 曾灶昌 王启平[3] 李东 徐玉红[4] 谢守霞 原文鹏 陈日来 李玉珍[4] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院,广东深圳518020 [2]深圳市光明新区公明医院,广东深圳518020 [3]深圳市沙井人民医院,广东深圳518020 [4]深圳市第四人民医院,广东深圳518020 [5]深圳市梅林医院,广东深圳518020

出　　处：《中南药学》2009年第7期484-486,共3页Central South Pharmacy

基　　金：深圳市科技计划项目(医疗卫生类);(编号:200802087)

摘　　要：目的建立柱前衍生化HPLC/UV法测定黄杨宁片中环维黄杨星D的含量。方法异氰酸苯酯为柱前衍生化试剂,流动相：甲醇-水（83∶17）;ZORBAX SB-C18（250 mm×4.6 mm,3.5μm）柱分离;检测波长242 nm;流速：1 mL.min-1;柱温：30℃;进样量：10μL。结果环维黄杨星D在2～400μg.mL-1与峰面积呈良好线性关系,回归方程为：Y=2 616X＋198.2（r=0.999 9）;环维黄杨星D平均回收率为102.6%,RSD为0.4%（n=6）;结论该法简单、准确度高、灵敏度好,为黄杨宁片质量控制提供了依据。Objective To establish a pre-column derivatization of HPLC/UV method for the content determination of cyclovirobuxine D in Huangyangning tablets. Methods A pre-column derivation was developed by HPLC with phenylisocyanate. A ZORBAX SB-Cx8 column （250 min×4. 6 mm, 3. 5μm） was used and the mobile phase contained a mixture of methanol-water （83 ： 17）, with a flow rate at 1.0 mL ·min-1. The column temperature was 30℃. The UV detection wavelength was 242 nm. Results The calibration curve of cyclovirobuxine D was linear at 2～400μg·mL （r=0. 999 9）. The average recovery was 102.6%, with RSD of 0. 4% （n=6）. Conclusion The method is simple, accurate, sensitive and suitable for the quality control of Huangyangning tablets.

关 键 词：柱前衍生化 环维黄杨星D 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]