高效液相色谱法快速测定卷柏药材中穗花杉双黄酮含量  

Rapid detemination of amentoflavone in Selagingella by HPLC

作　　者：张建华[1] 杨帆[2] 申健[2] 李福双[2] 刘建锋[2] 谭桂山[2] 徐康平[2] 

机构地区：[1]湖南省东安县疾病预防控制中心,湖南永州425900 [2]中南大学药学院,长沙410013

出　　处：《中南药学》2009年第7期487-489,共3页Central South Pharmacy

基　　金：国家自然科学基金资助项目(编号:30873149);中南大学贵重仪器共享基金资助项目(编号:ZKJ2008046)

摘　　要：目的建立中药卷柏中特征性成分穗花杉双黄酮的快速含量分析方法,比较其在不同卷柏药材中的含量差异。方法采用岛津VP-ODS色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.2%醋酸水溶液,梯度洗脱,流速为1.2 mL.min-1,检测波长为338 nm,柱温为30℃,进样量10μL。结果穗花杉双黄酮在0.295～8.853μg线性关系良好（r=0.999 99）,平均加样回收率为99.34%,RSD为0.94%（n=6）,其在卷柏药材中的含量为2.176～8.186 mg.g-1。结论穗花杉双黄酮在不同产地、不同品种卷柏中差异较大,本试验为卷柏药材质量评价和优良品种筛选提供简便快捷的方法。Objective To establish a rapid HPLC method for the determination of amentoflavone in 4 herbs of Selagin-gella Spring. Methods HPLC analysis was carried out on SHIMADZU VP-ODS （ 4.6 mm×250 mm, 5 μm ） at 30 ℃. The mobile phase was ACN-0.2%HAc. The flow rate was 1.2 mE·min^-1. The detection wavelength was 338 nm; Results The linear range of amentoflavone was 0. 295～8. 853μg （r= 0. 999 99 ）, the average recovery of this method was 99.34%, and the RSD was 0. 94% （n=6） . The content of amentoflavone in different samples was at 2. 176～8. 186 mg·g-1. Conclusion The contents of amentoflavone are obviouslly different in herbs of Selaginella with different localities and species. This quantitative measurement method provides a simple and rapid way for the quality control and species selection.

关 键 词：卷柏 穗花杉双黄酮 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R284.1