双孢蘑菇耐温差异蛋白质组学研究  

Differential Proteomics Studies on Thermotolerance of Agaricus bisporus

作　　者：陆兆明[1] 徐祯[1] 王珂[1] 陈澄[1] 陈岑渌璐[1] 宋思扬[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2009年第4期590-593,共4页Journal of Xiamen University：Natural Science

基　　金：国家基础科学人才培养基金(J0630649);国家自然科学基金(30570254)资助

摘　　要：利用蛋白质组学和荧光定量PCR方法,研究双孢蘑菇(Agaricus bisporusLange(Imbach))02菌株在常温与高温胁迫下的蛋白质表达差异,发现了6个差异蛋白质点,经NCBI数据库比对分析,表明6个差异蛋白质分别为HSP70家族的HSS1热激蛋白、异柠檬酸裂解酶、细胞色素P450单氧化酶及3个未知蛋白质.这些蛋白质在双孢蘑菇受热胁迫下具有保护自身稳定的功能.Agaricus bisporus is a kind of cultivated edible mushroom which has high economic value and important ecology significance. Environment temperature is the main restrained factor in its development, and this greatly limits its cultivation season. In this study,2D-eletrophoresis and real-time PCR are employed to discover the proteomic difference in Agaricus bisporus 02 strain after high temperature induction, and 6 differential expressed protein spots were finally obtained. Compared with the NCBI data base , spot 1 is the heat shock protein ,spot 2 is isocitrate lyase and spot 3 is cytochrome P450 monooxygenase. The other three spots are novel. Presumably,those proteins confer the thermotolerance on strain 02 under high temperature.

关 键 词：双孢蘑菇 双向电泳 热胁迫 蛋白表达差异 

分 类 号：Q935[生物学—微生物学]