中药何首乌饮对衰老大鼠卵巢组织胰岛素样生长因子Ⅰ及胰岛素样生长因子结合蛋白3表达的影响  

Effect of Traditional Chinese Medicine Heshouwuyin on the Expression of IGF-1 and IGFBP3 in the Ovary of the Aging Model Rats

作　　者：张娜[1] 李亚丽[2] 高福禄[3] 

机构地区：[1]河北医科大学第四医院,河北石家庄050011 [2]河北省人民医院,河北石家庄050071 [3]河北师范大学,河北石家庄050017

出　　处：《时珍国医国药》2009年第7期1569-1571,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30572432)

摘　　要：目的探讨何首乌饮抗大鼠卵巢组织衰老的机理,为抗女性性腺的衰老研究提供理论指导和实验依据。方法将60只SD大鼠随机分为3组:正常组、模型组、预防组。采用D-半乳糖连续腹腔注射法制造雌性大鼠亚急性衰老模型,预防组在造模的同时以中药方剂何首乌饮灌胃。应用原位杂交方法检测各组大鼠卵巢组织胰岛素样生长因子Ⅰ(IGF-Ⅰ)蛋白的表达,用RT-PCR方法检测各组大鼠卵巢组织胰岛素样生长因子结合蛋白3(IGFBP3)基因的表达情况。结果何首乌饮明显抑制了衰老大鼠卵巢组织中IGFBP3基因表达的上调趋势(P<0.05),且明显提高了衰老大鼠卵巢组织中IGF-Ⅰ蛋白的表达(P<0.05)。结论何首乌饮通过影响衰老大鼠卵巢中细胞因子IGF-Ⅰ、IGFBP3的表达,起到抗大鼠性腺衰老的作用。Objectlve To study the mechanism of traditional Chinese medicine Heshouwuyin（HSWY） in anti -aging of the aging model rat ovary, so as to provide the oretical and experimental evidence to anti - aging in the female sexual gland. Methods 60 rats were divided into three groups at random： normal group, aging model group, treatment group. The rats in treatment group were redivided into four groups： HSWY low dosage group, HSWY middle dosage group, HSWY high dosage group and Heshouwan（HSWW） group. The sub -acute aging model rats were induced by D -galactose, and the rats in treatment group were ad- ministered intragastrieaug with HSWY and HSWW. The gene expression of insulin growth factor banding protein 3 （ IGFBP3 ） in ovary of each group was detected reverse transcription polymerase chain reaction, and the protein expression of insulin growth factor - 1 （ IGF - 1 ） was examined by the method of in - situ hybridization. Results The gene expression of IGFBP3 was up - regulated and the protein expression of IGF - 1 were inhibited in ovaries of aging rats. HSWY significantly suppressed the gene expression of IGFBP3 （ P 〈 0.01 ） and increased the protein expression of IGF - 1 （ P 〈 0.01 ）. Conclusion HSWY has anti - aging effect on rat sexual gland through regalating the expression of cytokines such as IGF - 1 and IGFBP3 in ovary.

关 键 词：何首乌饮 衰老 大鼠 胰岛素样生长因子Ⅰ 胰岛素样生长因子结合蛋白3 RT-PCR 原位杂交 

分 类 号：Q95-3[生物学—动物学] R212.7[医药卫生—中医学]