六月青含药血清对HepG2.2.15细胞系HBsAg与HBeAg表达的影响  

The Inhibitory Effect of the Serum Containing Liuyueqing on the Expression of HBsAg and HBeAg in the HepG2.2.15 Cells

作　　者：林兴[1] 黄权芳[2] 张士军[1] 刘曦[1] 黄仁彬[1] 

机构地区：[1]广西医科大学,广西南宁530021 [2]广西中医学院第一附属医院,广西南宁530021

出　　处：《时珍国医国药》2009年第7期1603-1604,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广西教育厅自然科学基金项目(No.200710MS014)

摘　　要：目的观察六月青(Liuyueqing,LYQ)含药血清的体外抗乙型肝炎病毒的作用。方法以MTT法检测药物在体外对HepG2.2.15细胞的生长抑制作用,采用酶联免疫法(ELISA法)检测HepG2.2.15细胞上清液中HBsAg、HBeAg的滴度。结果LYQ含药血清对HepG2.2.15细胞50%生长抑制率的给药剂量为11.56g.(kg.d)-1;而对HepG2.2.15细胞分泌HBsAg50%生长抑制率的给药剂量为0.178g.(kg.d)-1,其治疗指数(TI)为64.94;另外,LYQ含药血清对HBeAg抑制作用不明显。结论LYQ含药血清在体外有显著的抗HBV的作用,且毒性较低。Objective To observe the effect of the serum containing Liuyueqing （LYQ） on the expression of HBsAg and HBeAg in HepG2.2.15 cells. Methods The toxicity of the serum containing LYQ on HepG2.2.15 cells was detected by means of MTT. The inhibitory effects of the serum containing LYQ on the excreation of HBsAg and HBeAg in HePG 2.2.15 cells were evaluated with method of enzyme linked immunosorbent assay （ELISA）. Results The dose which the serum containing LYQ could inhibit 50% HepG2.2.15 cells was 11.56 g. （ kg. d） - 1. The dose which the serum containing LYQ could inhibit 50% HBsAg was 0. 178g. （kg. d） - 1, and its treatment index （TI） on HBsAg was 64.94. The inhibitory effect of the serum containing LYQ on HBeAg was not obvious. Conclusion The serum containing LYQ can inhibit HBV significantly in vitro, and it is a low toxic drug.

关 键 词：六月青含药血清 HEPG2.2.15细胞 HBSAG HBEAG 

分 类 号：R285.5[医药卫生—中药学]