川芎嗪对急性百草枯中毒大鼠一氧化氮和诱导型一氧化氮合酶的影响及其治疗作用  

Effect of Ligustrazine on NO and iNOS in Paraquat-induced Acute Lung Injury in Rats

作　　者：刘会芳[1] 赵燕燕[2] 崔玉英[1] 陈春生[2] 

机构地区：[1]河北大学医学部卫生职业技术学院,河北保定071000 [2]河北大学医学实验中心,河北保定071000

出　　处：《时珍国医国药》2009年第7期1631-1632,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河北省中医药管理局科研基金资助项目(No.2008070;2008072);河北省卫生厅科研基金资助项目(No.08370);河北省科技攻关项目(No.05276101D-88;42761220);河北大学医学部科学研究基金资助项目(No.2007Q06)

摘　　要：目的观察百草枯(PQ)急性中毒大鼠所致肺损伤(ALI)时一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)的变化,探讨川芎嗪对急性百草枯中毒所致肺损伤的保护作用。方法将50只SD大鼠随机分成5组,空白组、阴性对照组、阳性对照组、川芎嗪低剂量组和川芎嗪高剂量组。观察大体标本,组织病理以及生物学标志:肺湿/干重比、肺泡灌洗液中性粒细胞比和蛋白含量。同时测定肺组织NO含量和iNOS活性。结果与阴性对照组相比,川芎嗪低剂量组肺组织病理显示肺淤血、肺水肿明显减轻。其生物学标志均降低(P<0.05或P<0.01),NO和iNOS也降低(P<0.01)。结论NO及iNOS在百草枯所致大鼠肺损伤中起重要作用,川芎嗪能降低NO及iNOS水平,减轻百草枯中毒大鼠肺组织损伤。Objective The variance of NO and iNOS in Paraquat - induced acute lung injured rats was investigated, and the protection of ligustrazine on rat acute lung injury was approached. Methods 50 Sprague - Dawley （SD） rats were randomly divided into five experimental groups： blank group, negative control group, positive control group, ligustrazine low- dose group（ LDG）, ligustrazine highdose group （HDG）. Macroscopic and histopathological examinations were performed and biological indexes were measured for the lung specimens. The indexes included lung wet weight/dry weight, the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid. In the mean time, the contents of lung tissue homogenate NO and iNOS were measured. Results Compared with negative control group, lung congestion and lung edema of ligustrazine low - dose group were miti- gated. Lung wet weight/dry weight, neutrophil infiltration, and protein content were decreased （ P 〈 0.05 or P 〈 0.01 ）, and bio- logical indexes of lung injury and the contents of NO and iNOS were significantly decreased （P 〈 0.05 or P 〈 0.01 ）. Conclusion NO and iNOS play important roles in lung injury of poisoned rats. Lignstrazine can ameliorate the lung injury and decrease the concentrations of NO and iNOS.

关 键 词：川芎嗪 百草枯 肺损伤 一氧化氮 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]