毛细管气相色谱法测定消肿止痛酊中樟脑与薄荷脑的含量  

Determination of Camphor and Menthol in Xiaozhongzhitong Tincture by Capillary Gas Chromatography

作　　者：张晓霞[1] 陈安家[1] 张一鸣[1] 王洁[1] 李霞[1] 李进冉[1] 

机构地区：[1]山西医科大学药学院,山西太原030001

出　　处：《时珍国医国药》2009年第7期1645-1646,共2页Lishizhen Medicine and Materia Medica Research

基　　金：山西省自然科学基金(No.2008011077-1)

摘　　要：目的建立测定消肿止痛酊中樟脑和薄荷脑含量的方法。方法采用毛细管气相色谱法。色谱柱为PEG-20M石英毛细管柱(30m×0.25mm×0.25μm);载气为氮气;正辛醇为内标;柱温160℃;氢火焰离子检测器;检测器和气化室的温度均为250℃;N2流速3ml/min;H2流速30ml/min;空气流速300ml/min;尾吹气N2流速30ml/min。结果樟脑、薄荷脑和正辛醇能达到良好分离;樟脑的线性范围3.00～18.00μg/ml(r=0.9992),薄荷脑的线性范围3.20～19.20μg/ml(r=0.9995);樟脑的回收率98.5%～101.5%,薄荷脑的回收率97.6%～101.7%。结论方法简便、准确、专属性强、重复性好,可用于消肿止痛酊的质量控制。Objective To establish a method for GC determination of Camphor and Menthol in Xiaozhongzhitong Tincture. Methods Capillary GC was adopted. The internal standard method was employed with octanol as internal standard. The GC system consisted of a flame ionization detector, PEG -20M capillary column（30 m ×0.25 mm ×0.25 p.m）, nitrogen as the carrier gas; oven temperature 160℃, detector temperature 250℃, N2 ： 3 ml/min, n2 ： 30 ml/min, Air ： 300ml/min,made up（ N2 ） ： 30 ml/min. Results Camphor and Menthol could be separated well under the chromatographic conditions. The linear range of Cam- phor was 3.00 -18.00 μg/ml （ r = 0.999 2）, and the linear range of the Menthol was 3.20 -19.20 μg/ml （ r = 0. 999 5 ） ; the recovery of Camphor was 98.5% - 101.5%, and the recovery of Menthol was 97.6% - 101.7%. Conclusion The method is simple, sensitive, accurate and has good repeatability, and it can be used to control the quality of Xiaozhongzhitong Tincture.

关 键 词：毛细管气相色谱法 消肿止痛酊 樟脑 薄荷脑 

分 类 号：R284.2[医药卫生—中药学]