扶正利湿活血复方对鸭胚肝细胞中鸭乙型肝炎病毒的抑制作用  

Inhibitory Effect of Chinese Medicine Compound Fuzheng Lishi Huoxue on Duck Hepatitis B Virus (DHBV) in Duck Embryo Liver Cells

作　　者：刘汉平[1] 何金洋[2] 梁波[3] 

机构地区：[1]华南师范大学光子中医学实验室,广东广州510631 [2]广州中医药大学热带病研究所,广东广州510405 [3]华南师范大学医院,广东广州510631

出　　处：《时珍国医国药》2009年第7期1670-1672,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广东省中医药局建设中医药强省科研课题(No.1060106)

摘　　要：目的探讨扶正利湿活血中药复方水提物和醇提物对先天感染乙肝病毒(HBV)的鸭胚肝细胞乙型肝炎病毒(DH-BV)的抑制作用。方法荧光定量PCR技术对鸭胚尿囊液DHBV DNA分析筛选先天感染DHBV的阳性鸭胚,对DHBV阳性的鸭胚肝细胞原代培养;细胞接种后第3天分组加入不同剂量扶正利湿活血复方水提物和醇提物,共6d,分别于药物干预前、药物干预第3天、第6天、停药第2天收集培养上清;辛酸钠法提取上清DHBV DNA,实时荧光定量PCR(FQ-PCR)检测分析血清DHBV DNA的含量。结果水提物各剂量组组内不同时间点DHBV DNA载量相比无显著性差异(P(0.05),仅水提物低剂量组DHBV DNA载量显著低于相同时间点病毒对照组(P<0.05);醇提物中、低剂量组在给药后和停药后培养上清DHBV均明显下降(组间、组内比较均为P<0.01),扶正利湿活血复方醇提物高剂量组在给药后第3天时DHBV明显下降(组间、组内比较均为P<0.01),第6天时组内比较无明显下降(P>0.05),而在停药后第3天时组内与组间比较又均有显著性差异(均为P<0.01)。结论扶正利湿活血复方醇提物对鸭胚肝细胞中的DHBV有直接抑制作用,水提物在鸭体内可能不是通过直接抑制DHBV而起作用。Objective To study the inhibitory effect of Chinese medicine compound prescriptions Fuzheng Lishi Huoxue on duck hepatitis B virus （DHBV） in duck embryo liver Cells. Methods The duck embryo liver cells with congenital infection DHBV were incubated with different concentration extracts of Chinese medicine compound Fuzheng Lishi Huoxue, which was extracted with water and alcohol respectively. The level of DHBV DNA in culture supernatants was detected using FQ - PCR at different time points after the cell being inoculated. Results Water extract of Fuzheng Lishi Huoxue had no significant effect on supernantant DHBV DNA Content （ P 〉 0.05 ）, but alcohol extract of Fuzheng Lishi Huoxue supernatant DHBV DNA content （ P 〈 0. 01 ）. Conclusion The alcohol extract of Chinese medicine Compound Fuzheng Lishi Huoxue has direct inhibitory effect on duck hepatitis B virus （DHBV） in duck embryo liver cells.

关 键 词：扶正利湿活血 鸭乙型肝炎病毒 肝细胞 荧光定量PCR 

分 类 号：R285.5[医药卫生—中药学]