离子交换层析纯化透明质酸  

Purification of hyaluronic acid by ion exchange chromatography

作　　者：刘骥翔[1] 叶华[1] 苏海佳[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《生物加工过程》2009年第4期32-35,共4页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20636010;20876011);国家高技术研究发展计划(863计划)资助项目(2006AA02Z245;2007AA100404)

摘　　要：考察6种离子交换树脂的静态吸附解析效果,选出201*7阴离子交换树脂填柱,确定洗脱流速为0.6mL/min,40mL0.3mol/LNaCl和50mL0.5mol/LNaCl双浓度洗脱,实现透明质酸和杂蛋白的分离。制得透明质酸产品蛋白含量为0.057%,葡萄糖醛酸含量为43%,平均相对分子质量大于1.1×106,收率为54%,符合医用级透明质酸行业标准的要求。A chromatography method was developed to purify hyaluronic acid （ HA ） of medical usage. Strong acidic cation exchange resin （ brand 201 ＊ 7） was selected for the separation. The optimal elute rate was 0.6 mL/min. The optimal concentrations of eluents were 0. 3 mol/L NaCl, and 0. 5 mol/L NaCl with the optimal elute volumes of 40 mL and 50 mL, respectively. The protein content of the purified HA was 0. 057% and the molecular weight was more than 1.1 × 10^6. The content of glucuronic acid was 43%. The yield of HA reached 54%. The purified HA reached the industry standard of medical HA.

关 键 词：离子交换层析 纯化 透明质酸 

分 类 号：Q539[生物学—生物化学]