产唾液酸酶微生物的筛选及产酶条件的优化  

Screening of sialidase-producing microorganism and optimization of sialidase production

作　　者：曹丹[1] 王学东[1] 张鲁嘉[1] 张建国[1] 魏东芝[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《生物加工过程》2009年第4期40-45,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家高技术研究发展计划(863计划)重点资助项目(2009ZX09306-001)

摘　　要：以无单唾液酸四己糖神经节苷脂(GM1)的神经节苷脂为唯一C源,从土壤中筛选出1株能以多唾液酸神经节苷脂为底物,转化生成GM1的产唾液酸酶微生物,经鉴定为溶黄嘌呤厄菌(Oerskovia xanthineolytica)。利用单因素法和响应面分析法对溶黄嘌呤厄菌产生唾液酸酶的条件进行优化,酶活提高了4.89倍。利用优化后培养条件,以神经节苷脂混合体为底物,经过微生物生物转化后,单唾液酸神经节苷脂GM1的含量从10%提高到83.7%。Oerskovia xanthineolytica--the strain which used to convert polysialoganglioside into monosialotetrahexosyl ganglioside （GM1） was efficiently screened from soil through high flux screening of sialidaseproducing bacterium with free GM1 gangliosides as the sole source carbon. The concentration for maximum enzyme activity was optimized by single factor response surface analysis. The strain was cultured in the optimized m gangliosides are edium containing crude gangliosides （ 10% w/v） at 37℃ for 12 h. Most of the polysialo converted into GM1. The relative content of GM1 increased from 10% to 83.7% in the crude gangliosides.

关 键 词：唾液酸酶 生物转化 单唾液酸四己糖神经节苷脂 溶黄嘌呤厄菌 

分 类 号：Q546[生物学—生物化学] O629.1[理学—有机化学]