山东汉族RhD阴性个体基因多态性研究  

Study of RhD gene polymorphism among RhD-negative Han population in Shandong province

作　　者：浑守永[1] 

机构地区：[1]山东大学附属省立医院输血科,山东济南250021

出　　处：《检验医学》2009年第7期493-496,共4页Laboratory Medicine

摘　　要：目的研究山东汉族人群RhD阴性个体RhD基因多态性。方法采用标准血清学方法和抗球蛋白实验筛选Rh阴性个体;对Rh阴性个体再用吸收放散实验确定DEL型。运用多重聚合酶链反应(PCR)方法分析RhD阴性个体基因存在情况。结果67例Rh阴性个体中DEL型16例,占Rh阴性个体的23.88%,其6个RhD基因特异性的第3、4、5、6、7、9外显子全部存在;剩下51例Rh阴性个体中1例缺失第5外显子,其余50例6个外显子全部缺失。结论山东汉族人群RhD阴性个体中存在一定比例的DEL型,且所有DEL样本均具有完整的RhD基因,在排除DEL型后的RhD阴性个体可能携带RhD基因,但其比例较低。Objective To study the RhD gene polymorphism among RhD-negative Han population in Shandong province. Methods The RhD-negative individuals Were screened by the standard serological method and antiglobulin test ; The DEL individuals were identified by absorption/elution method. RhD gene polymorphisms in RhD-negative individuals were analyzed by multiplex polymerase chain reaction （PCR）. Results 16 of 17 individuals were identified as DEL and the percentage of DEL was 23.88%. All DEL individuals had all the six RhD-specific exons （Exon 3, 4, 5, 6, 7 and 9）. The RhD-negative individuals showed two polymorphisms： 1 of 51 individuals lacked the fifth exon and the others of them lacked all the six RhD-specific exons. Conclusions DEL phenotype is highly detected among RhD-negative Han population in Shandong province. DEL individuals have intact RhD gene . The RhD-negative individuals excluding DEL phenotyping may carry RhD gene. However, the carrying proportion is low.

关 键 词：RH血型系统 RHD基因 多态性 聚合酶链反应 多重 

分 类 号：Q503[生物学—生物化学]