人类免疫缺陷病毒合并结核感染患者外周血Vγ2Vδ2^＋T淋巴细胞的数量与功能变化  

Impaired Vγ2Vδ2^＋T lymphocytes responses during Myeobacterium tuberculosis infection in human immunodeficiency virus infected individuals

作　　者：虞胜镭[1] 邵凌云[1] 金嘉琳[1] 张舒[1] 张文宏[1] 陈澍[1] 

机构地区：[1]复旦大学附属华山医院感染科,上海200040

出　　处：《中华传染病杂志》2009年第7期413-417,共5页Chinese Journal of Infectious Diseases

基　　金：国家科技重大专项课题资助项目（2008Zx10302）

摘　　要：目的探讨外周血Vγ2Vδ2^＋T淋巴细胞数量与功能变化对HIV合并结核感染状态的影响。方法将76例HIV／AIDS合并结核感染患者分为活动性结核感染组（HIV＋TB组）和潜伏结核感染组（HIV＋LTB组）。流式细胞仪测定外周血淋巴细胞分类情况。酶联斑点免疫法（ELISPOT）和胞内细胞因子染色（ICS）方法检测在PPD和磷酸化抗原（HMBPP）的刺激下T淋巴细胞亚群分泌IFN-γ功能的情况。统计学处理采用t检验。结果HIV＋TB组CD3^＋T淋巴细胞绝对计数（t=-3．67，P〈0．01）和Vγ2Vδ2^＋T淋巴细胞所占CD3^＋T淋巴细胞比例（t=-2．06，P〈0．05）均显著低于HIV＋LTB组。PPD刺激时，HIV＋LTB组分泌特异性IFN-γ的T淋巴细胞和参与分泌IFN-γ的CD4^＋T淋巴细胞所占CD3^-T淋巴细胞比例，与HIV＋TB组比较差异均无统计学意义。HMBPP刺激时，HIV＋LTB组与HIV＋TB组比较，分泌IFN-γ的特异性T淋巴细胞计数（t=2．71，P〈0．01）和产生IFN7的特异性Vγ2^＋T淋巴细胞比例（t=3．003，P〈0．01）均显著增加。结论Vγ2Vδ2^＋T淋巴细胞在HIV／AIDS合并活动性结核患者中的数量和功能都有受损，提示该类细胞在CD4^＋T淋巴细胞受到抑制的情况下可能是人体内抵御结核感染的重要免疫细胞。Objective To evaluate the number and function of peripheral Vγ2Vδ2^＋T lymphocytes during Mycobacteriurn tuberculosis （MTB） infection in human immunodeficiency virus （HIV） infected individuals. Methods Seventy-six HIV/acquired immune deficiency syndrome （AIDS） patients co-infected with MTB were divided into active tuberculosis （TB） group （HIV＋ TB） and latent TB group （HIV＋LTB）. T cell subsets of peripheral blood lymphocytes were analysed by flow cytometry. Stimulated by protein purified derivative （PPD） and hydroxymethylbutenyl diphosphate （HMBPP）, specific interferon （IFN）-γ producing T cells were detected using enzyme-linked immunospot （ELISPOT） and intraeellular cytokine staining （ICS）. Data were analyzed by t test. Results The absolute number of CD3^＋ T cells and proportion of Vγ2Vδ2^＋T ceils in CD3^- T cells in HIV＋TB group were both significantly lower than those in HIV＋LTB group （t=-3.67, P〈0.01 ; t=-2.06, P〈0.05）. PPD-specific IFN-γ-producing T cells and percentage of PPD-specific CD4^- T cells in CD3^＋ T cells in HIV＋LTB group were both similar with those in HIV＋TB group. While HMBPP-specific IFN-γ-producing T cells and percentage of HMBPP-specific Vγ2^＋ T cells in CD3^＋ T cells in HIV＋LTB group were both higher than those in HIV＋TB group （t=2. 71 and t=3. 003, respectively; both P〈0. 01 ）. Conclusion The number and function of Vγ2Vδ2^＋T cells were impaired in HIV/AIDS patients coinfected with active MTB infection, which indicates that Vγ2Vδ2^＋T cells may be the key immune cells against MTB in individuals with impaired CD4^- T cells.

关 键 词：Vγ2Vδ2^+T淋巴细胞 结核 HIV感染 

分 类 号：R686[医药卫生—骨科学]