慢性乙型肝炎患者外周血及肝组织中CD4^＋CD25^＋调节性T细胞和乙型肝炎病毒特异性细胞毒性T淋巴细胞的表达及意义  

The presence of CD4^＋ CD25^＋ regulatory T cells and hepatitis B virus specific cytotoxic T lymphocyte in peripheral blood and liver tissues of patients with chronic hepititis B and its significance

作　　者：裴豪[1] 陆忠华[1] 钱金娟[1] 杨小娟[1] 蒋祥虎[1] 曹立森[1] 

机构地区：[1]无锡市传染病医院检验科,214005

出　　处：《中华传染病杂志》2009年第7期431-434,共4页Chinese Journal of Infectious Diseases

摘　　要：目的研究CD4^＋CD25^＋调节性T细胞和HBV特异性CTL在慢性乙型肝炎患者外周血和肝组织中的表达和临床意义。方法流式细胞分析技术和流式细胞术细胞因子测定法（CFC）检测157例HBV感染者（包括急性乙型肝炎20例、慢性乙型肝炎115例、乙型肝炎肝硬化22例）和20例健康对照组外周血和部分肝组织中CD4^＋CD25^＋调节性T细胞和HBV特异性CTL的表达。组间分析采用t检验。结果急性乙型肝炎，慢性乙型肝炎轻、中、重度患者外周血中CD4^-CD25^＋调节性T细胞分别为（2．87±0．94）％、（3．53±1．56）％、（4．59±2．98）％和（3．65±1．73）％，明显高于对照组的（2．36±0．60）％（t值分别为2．04、5．97、3．30和3．17，P％0．01）；慢性乙型肝炎轻、中、重度和乙型肝炎肝硬化患者外周血HBV特异性CTL为（0．189±0．152）％、（0．103±0．110）％、（0．118±0．120）％和（0．098±0．101）Yo，明显低于急性乙型肝炎患者的（0．815±0．360）％（t值分别为10．09、11．87、9．17和8．96，P％0．01）。肝组织中CD4^＋CD25^-调节性T细胞和HBV特异性CTL的表达高于外周血。结论CD4^＋CD25^＋调节性T细胞可能通过抑制CD8^＋T淋巴细胞在机体抗病毒过程中发挥重要作用。Objective To study the presence of CD4^＋ CD25^＋ regulatory T cells and hepatitis B virus （HBV） specific cytotoxic T lymphocyte （CTL） in peripheral blood and liver tissues of patients with chronic hepititis B （CHB） and its clincial significance. Methods One hundred and fifty seven HBV infected patients, including 20 cases of acute hepatitis B, 115 cases of chronic hepatitis B, and 22 cases of HBV-related liver cirrhosis, and 20 healthy controls were enrolled in this study. Peripheral blood was collected and liver tissues were obtained from some of the enrolled subjects. The CD4^＋ CD25^＋ regulatory T cells and HBV specific CTL were analyzed using flow cytometry and cytokine flow cytometry （CFC）. The comparison between groups was done by t test. Results The percentages of CD4^- CD25^＋ regulatory T cells in the peripheral blood of patients with acute hepatitis B and CHB of mild, moderate and severe degree were （2. 87±0. 94）%, （3. 53±1. 56）%, （4. 59±2. 98）% and （3.65±1.73）%, respectively, which were higher than that of controls （2.36±0.60）% （t=2.04, 5.97,3.30 and 3.17,respectively,P〈0.01）. The percentages of HBV specific CTL in the peripheral blood of patients with mild, moderate and severe degree of CHB and HBV related liver cirrhosis were （0.189±0.152）%,（0. 103±0. 110）%,（0. 118±0. 120） and （0.098±0. 101）%, respectively, which were significantly lower than that of acute hepatitis patients [ （0.815 ± 0. 360）% ] （t = 10.09, 11.87,9.17 and 8.96,respectively,P〈0.01）. CD4^＋ CD25^＋ regulatory T cells and HBV specific CTL in liver tissues were both higher than those in the peripheral blood. Conclusion CD4^-CD25^＋ regulatory T cells may play an important role in anti-HBV immune response through inhibiting CD8^ T cell function.

关 键 词：肝炎 乙型 慢性 CD4阳性T淋巴细胞 T淋巴细胞 调节性 T淋巴细胞 细胞毒性 肝 

分 类 号：R686[医药卫生—骨科学]