高脂血症apoE基因敲除小鼠心脏中一氧化氮合酶含量及基因表达  

Content and gene expression of eNOS in heart of hyperlipidemia apoE-/-mice

作　　者：曹晓娥 李晓波[2] 殷莲华[2] 

机构地区：[1]上海职工医学院生理教研室,上海200237 [2]复旦大学上海医学院生理学与病理生理学系,上海200032

出　　处：《复旦学报（医学版）》2009年第4期403-406,共4页Fudan University Journal of Medical Sciences

摘　　要：目的探讨高脂饲料对apoE基因敲除(apoE-/-)小鼠心肌组织中内皮型一氧化氮合酶(endothelial nitric-oxide synthase,eNOS)的含量及基因表达的影响。方法将13只apoE-/-小鼠随机分为两组,即高脂饲料组(n=7)和普通饲料组(n=6)。喂养12周后,测血清总胆固醇(total cholesterol,TC)、三酰甘油(triglyceride,TG)、高密度脂蛋白(high density lipoprotein,HDL)和低密度脂蛋白(low density lipoprotein,LDL)含量,采用Real-Time RT-PCR法检测心肌组织中eNOS的mRNA表达水平,以免疫组化方法检测eNOS蛋白的表达。结果高脂饲料组TC、TG和LDL明显高于普通饲料组,HDL则低于普通饲料组(P<0.01);高脂饲料组心肌组织中eNOS的mRNA和蛋白水平均低于普通饲料组(P<0.01)。结论高脂饲料导致apoE-/-小鼠发生高脂血症,并引起心肌组织中eNOS的mRNA表达的减少和相应蛋白水平的降低。Objective To explore the content and gene expression of endothelial nitric-oxide synthase （eNOS） in heart of hyperlipidemia apoE-/- mice. Methods apoE-/- mice were fed with high fat diet as high-fat diet group （n= 7） and normal fat diet as control group （n = 6） for 12 weeks. Serum lipid profiles including total cholesterol （TC）, triglyceride （TG）, high density lipoprotein （HDL） and low density lipoprotein （LDL） were detected, eNOS mRNA expression in heart were determined by Real-Time RT-PCR, as well as protein expression by immunohistochemistry. Results Levels of serum TC, TG and LDL clearly increased in high fat diet group, while HDL decreased （P〈0. 01 ）. mRNA and protein expression of eNOS in heart reduced in high fat diet group（P〈0.01 ）. Conclusions High fat diet may lead to hyperlipidemia in apoE-/- mice, which may down-regulate eNOS expression and decrease eNOS content in heart.

关 键 词：高脂血症 内皮型一氧化氮合酶 APOE-/-小鼠 

分 类 号：R589.2[医药卫生—内分泌]