4种石杉科植物中产生石杉碱甲的内生真菌的分离及初步鉴定和HPLC测定石杉碱甲的含量  

Isolation and preliminary identification of the endophytic fungi which produce Hupzine A from four species in Hupziaceae and determination of Huperzine A by HPLC

作　　者：鞠錾[1] 王峻[2] 潘胜利[1] 

机构地区：[1]复旦大学药学院生药学教研室,上海200032 [2]嘉兴学院医学院药学系,嘉兴314001

出　　处：《复旦学报（医学版）》2009年第4期445-449,共5页Fudan University Journal of Medical Sciences

基　　金：福建三明明溪县与复旦大学合作基金项目

摘　　要：目的从4种石杉科植物中得到产生石杉碱甲(Huperzine A)的内生真菌,改进筛选内生真菌的培养条件。方法消毒后的材料在培养基上培养和分离其内生真菌,HPLC检测代谢产物,显微形态鉴定菌种。结果从蛇足石杉,长柄石杉,伏贴石杉,柳杉叶马尾杉中分离得到32株内生真菌,其中从柳杉叶马尾杉分离出的2株内生真菌能够产生石杉碱甲,分别是HA15.(芽胞菌属)和HA23.(葡萄孢属)。结论首次从柳杉叶马尾杉中分离出能产生石杉碱甲的内生真菌。Objective To obtain the endophytic fungi which produce Huperzine A from four species in Hupziaceae and improve the culture conditions of screening endophytic fungi. Methods Plant materials were cultivated in culture medium after sterilization and their endophytic fungi were isolated. Hupzine A from metabolic was determined by HPLC, and the strains were identified by microscopic features. Results Thirty-two endophytic fungi were isolated from Huperzia serrata （Thunb. ex Murray） Trev, H. serrata （Thunb. ex Murray） Trev var. longipetiolata （Spring） H. M. Chang, H. appressa （Desv.） LOve et D. LOve, Phlegmariurus cryptomerianus （Maxim.） Ching ex L. B. Zhang et H. S. Kung. Two of these strains were isolated from P. cryptomerianus, HA15 （Blastornyces sp. ） and HA23 （Botrytis sp. ）, which produced Huperzine A. Conclusions Endophytic fungi producing Hupzine A has been successfully isolated from P. cryptomerianus.

关 键 词：石杉 马尾杉 内生真菌 石杉碱甲 高效液相色谱法 

分 类 号：Q946[生物学—植物学]