卵泡液NO、VEGF、ET-1水平与IVF-ET妊娠结局关系探讨  

Evaluation of IVF-ET outcome by measuring the level of NO,VEGF and ET-1 in follicular fluid

作　　者：赵敏[1,2] 常才[3] 刘智[1] 王诚[4] 韩金兰[4] 严敬明[4] 

机构地区：[1]复旦大学附属妇产科医院妇科,上海200011 [2]无锡市妇幼保健院,无锡214002 [3]复旦大学附属肿瘤医院超声科,上海200032 [4]上海集爱遗传与不育诊疗中心,上海200011

出　　处：《复旦学报（医学版）》2009年第4期485-489,共5页Fudan University Journal of Medical Sciences

摘　　要：目的探讨卵泡液NO、VEGF、ET-1水平对IVF-ET妊娠结局的预测价值。方法对131例接受IVF-ET的不孕症患者,采用比色法测定其卵泡液内NO水平,ELISA检测其卵泡液内VEGF、ET-1水平。应用经阴道超声于控制性超排卵(COH)周期人绒毛膜促性腺激素(HCG)注射日进行卵巢体积、窦状卵泡数检测。结果131例患者中临床妊娠49例,妊娠率为37.40%。妊娠组卵泡液内NO、VEGF水平均高于未妊娠组,ET-1水平则低于未妊娠组(P<0.05)。HCG注射日,妊娠组总卵巢体积、窦状卵泡数均大于未妊娠组(P<0.05)。卵泡液内NO、VEGF水平与总卵巢体积、窦状卵泡数呈正相关。而卵泡液内ET-1水平与总卵巢体积、窦状卵泡数则呈负相关。结论卵泡液内NO、VEGF水平增高,ET-1水平降低,可能促进卵巢局部血流丰富,提高卵巢反应性,利于IVF-ET中妊娠成功。Objective To investigate the level of follicular fluid NO, VEGF and ET-1 in assessing IVF-ET outcome. Methods Totally 131 patients undergoing IVF ET cycles were recruited. The level of follicular fluid NO was measured by chromatometry. The follicular fluid VEGF and ET-1 were measured by ELISA. Transvaginal ultrasound was performed on human chorionic gonadotropin （hCG） injection day to determine ovarian volume and antral follicle count. Results The pregnancy rate was 37.40% （49/131）. There were significantly increased level of follicular fluid NO, VEGF and decreased level of follicular fluid ET-1 in the pregnant group than those in the non pregnant group （P〈0. 115）. Total ovarian volume and antral follicle count on HCG injection day were significantly higher in the pregnant group than those in the non pregnant group （P〈0.05）. The levels of follicular fluid NO and VEGF had positive correlations with the total ovarian volume and antral follicle count. However, the level of follicular fluid ET-1 had a negative correlation with the total ovarian volume and antral follicle count. Conclusions The high level of NO, VEGF and low level of ET-1 in follicular fluid are good predictors of ovarian blood flow and ovarian response in IVF-ET.

关 键 词：卵巢反应性 卵泡液 NO ET-1 VEGF 

分 类 号：R711.6[医药卫生—妇产科学]