槲皮素对胃癌MGC-803细胞VEGF-C及VEGFR-3表达水平的影响  

Effects of quercetin on the expression of VEGF-C and VEGFR-3 in human cancer MGC-803 cells

作　　者：于志君[1] 何丽娅[1] 陈勇[1] 吴勉云[1] 赵雪花[1] 王震宇[1] 

机构地区：[1]武汉科技大学医学院细胞与分子生物学研究所,湖北武汉430065

出　　处：《细胞与分子免疫学杂志》2009年第8期678-680,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：湖北省教育厅科研重点资助项目(D200511007)

摘　　要：目的:研究槲皮素抑制胃癌MGC-803细胞淋巴转移的作用。方法:实验分为对照组和40μmol/L槲皮素处理组两组。采用免疫组化和RT-PCR方法,检测槲皮素对VEGF-C及其受体VEGFR-3表达水平的作用。结果:槲皮素处理48h后VEGF-C和VEGFR-3表达水平均有下调,与对照组相比有统计学意义(P<0.01)。结论:槲皮素可下调胃癌MGC-803细胞VEGF-C、VEGFR-3的表达。AIM： To investigate the mechanism of quercetin on the inhibition of the lymphatic metastasis in human gastric cancer cells MGC-803. METHODS： Cells were divided into the control group and the quercetin（Que）-treated group. Imrnunohistochemistry and RT-PCR were used to detect the expression of vascular endothelial growth factor C （VEGF-C） and VEGFR-3 of human gastric cancer cells MGC-803 in response to Que. RESULTS： Que significantly decreased the expression of VEGF-C and VEGFR-3 at 40 umol/L compared with the control group after 48 h （ P 〈 0. 01 ）. CONCLUSION： Que can down-regulate the expression of VEGF-C and VEGFR-3 in human gastric cancer cells MGC-803.

关 键 词：槲皮素 人胃癌MGC-803细胞 VEGF—C VEGFR-3 淋巴转移 

分 类 号：R33[医药卫生—人体生理学]