抗-HCV不同试剂检测结果分析  

Detection of anti-HCV antibody with different methods

作　　者：胡京辉[1] 张磊[1] 孙莉[1] 

机构地区：[1]北京市血液中心,100088

出　　处：《军医进修学院学报》2009年第4期498-499,共2页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的探讨不同检测方法、试剂对丙型肝炎病毒(HCV)抗体检测结果的影响。方法以不同试剂采用酶联免疫吸附试验(ELISA)对献血者血浆标本进行抗-HCV检测,呈反应性标本采用HCV RNA RT-PCR荧光定量确证检测。结果ELISA再次检测抗HCV抗体阳性率明显低于初次检测阳性率,差异有统计学意义(P<0.01),确证试验阳性率低于初次检测和再次检测的阳性率,差异有统计学意义(P<0.05)。结论ELISA测定结果为HCV抗体阳性者应经HCV RNA RT-PCR荧光定量加以确认。Objective To investigate the impact of different methods and reagents on detection of anti-HCV antibody in blood donors. Methods Anti-HCV antibody in blood donors was detected by enzyme-linked immunosorbent assay （ELISA） using different reagents, and positive sera were examined by Western blotting confirmatory test. Results Western blotting confirmatory test showed a lower positive anti-HCV rate than ELISA （P〈0.05）. Conclusion Positive HCV antibody detected by ELISA should be confirmed by quantifying fluorescence in HCV RNA RT-PCR.

关 键 词：肝炎 丙型 慢性 肝炎抗体 丙型 酶联免疫吸附测定 

分 类 号：R446.6[医药卫生—诊断学]