七氟醚对大鼠缺血再灌注心肌凋亡相关基因表达的影响  

Effected of sevoflurane pretreatment on myocardial Bcl-2 and Bax gene expression during ischemia/reperfusion in rats

作　　者：陈根殷[1] 王旭光[2] 杨展[2] 陈淑琼[1] 

机构地区：[1]广东医学院附属医院麻醉科,524001 [2]广东医学院附属医院中心实验室,524001

出　　处：《中国实用医药》2009年第9期1-3,共3页China Practical Medicine

基　　金：广东省卫生厅科研项目(项目编号:B2007143)

摘　　要：目的探讨七氟醚预处理对大鼠心脏缺血再灌注过程中心肌细胞Bcl-2及Bax基因表达的影响。方法24只SD大鼠随机分成3组(n=8):假手术对照组(C组)、缺血再灌注对照组(IR组)和七氟醚预处理组(s组)。IR组与S组接受左冠脉3h阻断和3h再灌注。七氟醚预处理组在缺血前吸入七氟醚,30min后洗脱15min。取心肌缺血区组织,RT-PCR测Bcl-2及Bax基因的mRNA表达,免疫印迹法(Western-blot)测蛋白表达。结果与C组相比较,IR组和S组Bcl-2的mRNA和蛋白表达均下调,而S组高于IR组;Bax的mRNA和蛋白表达IR及S组均高于C组,IR组则高于S组。结论七氟醚预处理抑制心肌细胞凋亡可能与上调Bd-2基因的表达、下调Bax基因的表达有关。Objective To investigate the effects of pretreatment with sevoflurane on myocardial Bcl-2 and Bax gene expression during ischemia-reperfusion in rats. Methods 24 SD rats were randomly divided into 3 groups with 8 animals in each group：sham operation group （group C） ; ischemia-reperfusion group （group IR）, sevoflurane group（group S）. In group IR animals were subjected to 3 h of ischemia by 3 h reperfusion. In group S animals inhales 2% sevoflurane for 30 min followed by 15 rain of wash-out before IR. In group C animals underwent no I/R. Myocardium was obtained from marginal zone of ischemic area. The mRNA and protein expression of Bcl-2 and Bax gene were detemined by RT-PCR and western-blot. Results The expression of mRNA and protein expression of Bcl-2 were upregulated in group S and group IR compared with that in group C. The expression of mRNA and protein expression of Bax were down-regulated in group S and group IR. The expression of Bcl-2 gene in group S was higher than that in group IR While the expression of Bax gene was lower. Conclusion Sevoflurane pretreatment inhibits myoeyte apoptosis in IR myocardium partly by modulation of expression of Bcl-2 and Bax genes.

关 键 词：七氟醚 心肌 心肌再灌注损伤 凋亡 

分 类 号：R541[医药卫生—心血管疾病]