沙蚕消化道D2菌株胞外蛋白酶抗肿瘤活性的初步探讨  

Preliminary study on anti-tumor activity of strain D2 protease from digestive tract of Perinereis aibuhitensis Grube

作　　者：徐莉莉[1] 王斌[1] 闫志勇[1] 钱冬萌[1] 李鹏[1] 郑旭[1] 

机构地区：[1]青岛大学医学院青岛市医药生物技术重点实验室,山东青岛266021

出　　处：《中国海洋药物》2009年第3期50-53,共4页Chinese Journal of Marine Drugs

基　　金：国家重大基础研究前期专项(2004CCA024000);青岛市科技专项(05-1-P-10);山东省科技发展计划项目(2004GG2205136);泰山学者工程资助

摘　　要：目的研究沙蚕消化道产蛋白酶菌D2株胞外蛋白酶(D2蛋白酶)的抗肿瘤活性。方法选用鼠乳腺癌细胞EMT6作为靶细胞,用不同浓度的D2蛋白酶,对体外培养靶细胞进行细胞增值抑制率检测,并与阳性对照组(紫杉醇组)和正常细胞对照组(人胚肺细胞组)比较。结果与结论沙蚕消化道产蛋白酶菌D2株胞外蛋白酶对鼠乳腺癌细胞EMT6存在增殖抑制作用,显示出一定的抗肿瘤活性。Objective To study the anti-tumor activity of strain D2 protease from digestive tract of Perinereis aibuhitensis Grube. Methods EMT6 cells were cultured in presence of protease at 5 concentration gradients for 5 different times and then MTT assay was used to evaluate the cell proliferation inhibition rate. Similarly, human embryonic lung cells （HELC） were under the same treatment with D2 protease and set up as normal cell control group. The effect of paclitaxel （PTX） on EMT6 cells was regarded as positive control group. Results and Conclusion The protease has inhibition effect on proliferation of EMT6 cells and shows antineoplastic activity in some degree.

关 键 词：海洋微生物 抗肿瘤药物 D2蛋白酶 

分 类 号：R915.1[医药卫生—微生物与生化药学] R965[医药卫生—药学]