促透剂对良肤乳膏中丹皮酚、白鲜碱、梣酮和甘草次酸的体外透皮吸收及皮肤滞留量的影响  

Effects of penetration enhancers on in vitro percutaneous absorption and amount retained in skin of paeonol,dictamnine,fraxinellone and glycyrrhetinic acid in Liangfu cream

作　　者：王森[1,2] 朱卫丰[1] 欧水平[2] 管咏梅[1] 陈丽华[1] 杨明[1,2] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004 [2]成都中医药大学,四川成都611173

出　　处：《中国中药杂志》2009年第14期1778-1782,共5页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2008BA153B07)

摘　　要：目的:研究不同促透剂对良肤乳膏中有效成分体外透皮吸收及皮肤滞留量的影响,筛选有效的促透剂。方法:选择氮酮、油酸、卡必醇、肉豆蔻酸异丙酯4种促透剂,采用改良的Franz扩散池法,以离体小鼠皮肤为透皮屏障,采用HPLC同时测定接受液和皮肤中有效成分的含量,考察不同促透剂对良肤乳膏中丹皮酚、白鲜碱、梣酮及甘草次酸的累积透过量、透皮吸收速率、时滞及皮肤滞留量的影响。结果:3%IPM对良肤乳膏中有效成分的促透效果较其他促透剂好,对丹皮酚、白鲜碱、梣酮、甘草次酸的促透倍数依次为1.52,1.24,1.73,3.21倍(P<0.05),并且对甘草次酸的皮肤滞留量增加倍数为1.96倍(P<0.05),对其他成分的滞留量影响不显著。结论:不同促透剂对良肤乳膏中不同有效成分的透皮吸收和皮肤滞留量的影响有差异,其中3%IPM既能促进良肤乳膏中4种有效成分的体外透皮吸收,又能增加甘草次酸的皮肤滞留量,可作为良肤乳膏的促透剂。Objective： To study the effects of different penetration enhancers on the in vitro percutaneous absorption and amount retained in skin of active ingredients in Liangfu cream and to screen out the effective accelerator. Method： Using improved Franz-type difusion cell and excised small mouse skin in vitro as transdermal barrier, the amount retained in skin and kinetics parame- ters of active ingredients such as cumulative permeation quantity, permeation rate and permeation lagged time were determined by HPLC. The enhancement ability of four different enhancers such as azone, oleic acid, transcutol P and isopropyl myristate were investi- gated. Result： 3% IPM enhanced the cumulative permeation quantity better than other penetration enhancers. The enhancive perme- ation multiples of paeonol,dictamnine,fraxinellone and glycyrrhetinic acid were 1.52, 1.24, 1.73 and 3.21 times （P 〈0. 05）. The enhancive amount retained in skin multiple of glycyrrhetinic acid was 1.96 times （P 〈 0. 05 ）, but for other components there were no significant impacts. Conclusion： The effects of penetration enhancers on the in vitro percutaneous absorption and amount retained in skin of components in Liangfu cream are different. 3% IPM which can enhance the cumulative permeation quantity of four components and amount retained in skin of glycyrrhetinic acid is the most suitable penetration enhancer for Liangfu cream.

关 键 词：促透剂 良肤乳膏 丹皮酚 白鲜碱 梣酮 甘草次酸 透皮吸收 皮肤滞留量 

分 类 号：R283[医药卫生—中药学]