益母草的化学成分及其抗人白血病K562细胞活性研究  

Chemical constituents and antitumor activity on leukemia K562 cell of Leonurus heterophyllus

作　　者：丛悦[1] 郭敬功[2] 王天晓[1] 李勉[1] 李克[1] 王金辉[3] 李钦[1] 

机构地区：[1]河南大学药学院中药研究所,河南开封475004 [2]河南大学植物逆境生物学重点实验室,河南开封475004 [3]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中国中药杂志》2009年第14期1816-1818,共3页China Journal of Chinese Materia Medica

基　　金：新世纪优秀人才支持计划项目(NCET-04-0289);河南大学校内基金项目(07YBZR037)

摘　　要：目的：研究益母草的化学成分,评价益母草化学成分体外抗白血病K562细胞活性。方法：运用硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、聚酰胺柱色谱和大孔树脂等色谱方法对益母草化学成分进行分离纯化,利用核磁共振技术鉴定了它们的化学结构。通过测试体外最低抑制浓度评价有关物质的抗肿瘤活性。结果：分离、纯化并鉴定了8个化合物,分别为槲皮素-3-O-洋槐双糖苷（quercetin 3-O-robinobioside,1）,芦丁（rutin,2）,异槲皮苷（isoquercitrin,3）,金丝桃苷（hyperoside,4）,槲皮素（quercetin,5）,芹菜素（apigenin,6）,芫花素（genkwanin,7）和苯甲酸（benzoic acid,8）。结论：化合物1,3,4,8为首次从该属植物中分离得到,化合物2,5~7首次从该种植物中分离得到。化合物1~6,8显示出不同程度的抑制人白血病K562细胞活性。The chemical constituents of Leonurus heterophylhts were separated and purified by repeated column chromatography on silica gel, HPD 100, Sephadex LH-20, and PHPLC. Each compound was characterized by spectroscopic and physical data. Eight compounds have been purified and identified to be quercetin 3-O-robinobioside （1）, rutin （2）, isoquerci trin （3）, hyperoside （4）, quercetin （5）, apigenin （6）, genkwanin （7） , and benzoic acid （8）. Among them,compounds 2, 5-7 were isolated from L. hetero- phyllus for the first time ; Compounds 1, 3, 4, 8 were obtained for the first time from the genus Leonurus. The in vitro activities against leukemia K562 Cells of pure components were evaluated by testing their IC50 Compounds 1-6, 8 exhibited in-vitro inhibitory activities against leukemia K562 cells in different extent.

关 键 词：益母草 化学成分 抗人白血病K562细胞活性 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]