分子印迹聚合物-固相萃取-高效液相色谱法测定地表水中莠去津  

HPLC Determination of Atrazine in Surface Water with SPE Packed with Molecularly Imprinted Polymer

作　　者：高会云[1] 何娟[1] 刘德仓[1] 吕瑞鹤[1] 卢奎[1] 

机构地区：[1]河南工业大学化学化工学院,郑州450001

出　　处：《理化检验（化学分册）》2009年第7期827-829,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省重大科技攻关(0422031200);郑州市科技攻关(064SGDG25127-8)资助项目

摘　　要：合成了莠去津分子印迹聚合物，制备了分子印迹固相萃取柱，用于水样中莠去津的分离和富集。对分子印迹固相萃取的萃取条件，如上样溶剂，淋洗溶剂，洗脱溶剂的种类及其用量等作了试验并予以优化。水样直接引入固相萃取柱中，然后用甲醇-水（1＋4）溶液淋洗。经抽干后，用甲醇作为洗脱剂将莠去津从柱上洗下，收集洗出液供高效液相色谱测定用。按所提出的分子印迹固相萃取柱（MIP-SPE）富集方法，对莠去津的富集倍数达82倍。方法的线性范围为1-200μg·L^-1，检出限（3S／N）为0．5μg·L^-1，对10μg·L^-1和100μg·L^-1莠去津的加标回收率分别为96．0％和97．2％，相对标准偏差（n=6）分别为1．8％和3．1％。The molecularly imprinted polymer （MIP＇s） of atrazine was synthesized and packed into the SPE column which was used for separation and enrichment of atrazine from the water samples. Conditions for the MIP- SPE, including solvents used for sample introduction, for use as eluant and their amounts required, were studied and optimized. Water sample was introduced directly onto the MIP-SPE column, and rinsed with CH3OH-H2O（1＋4）. After drying the column by suction, atrazine on the column was eluted with methanol, which was collected and used for HPLC determination. Multiple of enrichment of atrazine by the proposed MIP-SPE attained to a value of 82. Linearity range for the determination was between 1 to 200 μg·L^-1 and the detection limit （3S/N） found was 0. 5μg·L^-1. Test for recovery was made at concentration levels of 10μg·L^-1 and 100μg·L^-1 of atrazine added as standard solution, values of recovery found were 96.0% [RSD （n=6） 1.8%] and 97. 2% [RSD （n=6） 3. 1%] respectively.

关 键 词：高效液相色谱法 莠去津 分子印迹聚合物 固相萃取 地表水 

分 类 号：O652.63[理学—分析化学]