石油集输系统中微生物群落结构研究  

Bacterial Communities in Crude Oil Gathering and Transferring System

作　　者：刘永军[1] 郑昕[1] 金鹏康[1] 

机构地区：[1]西安建筑科技大学西北水资源生态与环境教育部重点实验室,陕西西安710055

出　　处：《微生物学杂志》2009年第3期25-31,共7页Journal of Microbiology

基　　金：陕西省教育厅项目(06JK267);西安市科技计划项目(YF07196)

摘　　要：采用16S rRNA基因克隆-变性梯度凝胶电泳分析方法研究了石油集输系统原油和油田产水中的微生物群落结构。变性梯度凝胶电泳图谱显示:油田产水中微生物群落远比原油中的菌群丰富。所有的油田水样和原油样本中都存在与Ochrobactrum sp.和Stenotrophomonas sp.相关的细菌;原油样本中检测出与Burk-holderia sp.、Brevundimonas sp.和Propionibacterium sp.相关的细菌,而这些细菌在油田水样中未检出;在油田水样中检出与Hippeasp.、Acidovorax sp.、Arcobacter sp.、Pseudomonas sp.、Thiomicrospira sp.、Brevibacterium sp.、Ti-ssierella sp.和Peptostreptococcus sp.相关的细菌,而这些细菌在原油样本中未检出。用古细菌特异性引物进行检测发现在油田水样中存在与Methanomicrobials和Methanosarcinales相关的产甲烷菌,而这些细菌在原油样本中未检出。在石油集输过程中,油田水样和原油中微生物群落的相似性分别为83.3%和88.2%,说明微生物群落结构较为稳定。Microbial community＇ s structure of crude oil and oil field production water from an oil gathering and trans- ferring system were studied adopting 16S rRNA gene cloning-denaturing gradient gel electrophoresis （DGGE） analy- sis. The atlas of DGGE showed that microbial communities in oilfield production water were far more richer than those in crude oil. All samples from oilfield production water and crude oil existed bacteria related to Ochrobacterium sp. and Stenotrophomoaas sp. While samples from crude oil bacteria related to Burkholderia sp. , Brevundimoaas sp, and Propionibacterium sp. were detected, but none from oilfield production water samples. Bacteria related to Hippea sp. , Acidovorax sp. , Arcobacter sp. , Pseudomonas sp. , Thiomicrospira sp. , Brevibacterium sp. , Tissierella sp. and Pep- tostreptococcus sp. were detected from oilfield water samples, but none from crude oil samples. It were found Metha- nomicrobials and Methanosarcinales methane-producing related bacteria existed in oilfield water samples in the determi- nation using paleo-bacterial specific primers, but none from crude oil samples. The similarity of microbial communities of oilfield water and crude oil samples was 83.3% and 88.2% respectively in the process of gathering and transferring suggested that the microbial community structure was fairly stable.

关 键 词：群落结构 石油集输系统 16S RRNA基因 原油 油田产水 

分 类 号：X703.3[环境科学与工程—环境工程]