PIAS2蛋白在不同病理分型的无精子症患者睾丸组织中的表达  

Expression of PIAS2 in testis tissues of patients with different pathological types of azoospermia

作　　者：冼志勇[1] 李飞[1] 邹亚光[2] 邓永键[3] 丁彦青[3] 毛向明[1] 

机构地区：[1]南方医科大学南方医院泌尿外科,广州510515 [2]南方医科大学南方医院口腔科,广州510515 [3]南方医科大学南方医院病理科,广州510515

出　　处：《第二军医大学学报》2009年第7期780-782,共3页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30772167);广州市科技计划项目(2008J1-C131)~~

摘　　要：目的:探讨PIAS2在不同生精功能状态的人睾丸组织中的表达情况。方法:对80例无精子症患者进行睾丸组织病理检查诊断,根据病理形态的不同分为:生精功能正常或基本正常(n=40)、精子发生低下(n=20)、唯支持细胞综合征(n=20)等。选择上述各型结构完整的睾丸组织,分别应用免疫组化法(SP)对PIAS2在不同生精功能状态的睾丸组织进行研究。结果:PIAS2在各级生精细胞多为强阳性表达,呈深棕黄色;PIAS2在间质细胞及支持细胞表达弱阳性表达或不表达。结论:PIAS2染色强弱与睾丸生精功能的强弱有一定关系,是一个潜在的判断睾丸生精功能的指标。Objective：To investigate the expression of PIAS2 in the testis tissues of patients with different spermatogenic functions. Methods： Eighty testicular biopsy samples from patients with azoospermia were subjected to pathological diagnosis and were divided into the following groups according to the pathological findings：normal or basically normal spermatogenesis group （n = 40）, hypo-spermatogenesis group （ HS, n = 20）, and sertoli celbonly syndrome group （ SCOS, n = 20 ）. Immunohistochemieal method （SP） was used to examine the expression of PIAS2 in the testis tissues with different spermatogenic functions. Results： PIAS2 was strongly positive （dark brown） in the spermatogenic cells of different functions. It was weakly positive or absent in the Leydig cells and Sertoli cells. Conclusion：The status of PIAS2 in the testicular tissues is associated with the spermatogenie functions,and it may serve as a potential parameter for predicting of spermatogenic function.

关 键 词：男性不育 无精子症 PIAS2 睾丸 活组织检查 免疫组织化学染色 

分 类 号：R698.2[医药卫生—泌尿科学]