从噬菌体展示肽库中筛选PreS1抗原的结合肽  

Selection of peptides bind to PreS1 antigen from a phage-displayed peptide library

作　　者：陈青青[1] 郑慧敏[1] 郭葆玉[1] 

机构地区：[1]第二军医大学药学院生化药学教研室,上海200433

出　　处：《第二军医大学学报》2009年第7期783-785,共3页Academic Journal of Second Military Medical University

摘　　要：目的:从噬菌体随机七肽库中筛选能与PreS1抗原特异性结合的多肽。方法:利用噬菌体展示技术,以PreS1抗原为靶分子,从随机七肽库中进行生物亲和筛选,ELISA鉴定阳性克隆。结果:对肽库进行3轮筛选后,通过ELISA和竞争抑制ELISA,获得了特异性的结合肽,测序结果显示噬菌体上的短肽有共同序列。结论:利用噬菌体展示技术成功筛选到了PreS1抗原的结合肽,为乙肝的治疗和诊断探索新的途径。Objective：To screen for peptides that specifically bind to PreS1 antigen from a phage-displayed peptide library. Methods： The PreS1 antigen was used as the target molecule to screen the binding peptide from the Ph. D. -7 peptide library with phage display technique, and the positive clones were identified by ELISA. Results： After three rounds of biopanning, the binding peptides were screened from the peptide library by ELISA and competitive inhibiting ELISA. Sequencing result showed that the binding peptides had high affinity and specificity. Conclusion： A peptide binding PreS1 antigen has been successfully obtained by screening the phage display library,which paves a way for the diagnosis and treatment of hepatitis B infection.

关 键 词：PRES1抗原 噬菌体展示肽库 结合肽 乙型肝炎 

分 类 号：R373.21[医药卫生—病原生物学]