癌基因D52家族新基因PC-1在前列腺癌细胞系LNCaP和C4-2B的G_2/M期高表达  

A New Gene PC-1 of Tumor Protein D52 Family Expresses Increasingly in G_2/M Phase During Cell Cycle of Prostate Carcinoma Cell Lines LNCaP and C4-2B

作　　者：武瑞琴[1] 李其满[1,2] 张惠[1] 俞岚[1] 周建光[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850 [2]福建农林大学,福建福州350002

出　　处：《生物技术通讯》2009年第4期455-457,共3页Letters in Biotechnology

基　　金：国家自然科学基金(30770834);国家高技术研究发展计划(2007AA02Z182)

摘　　要：目的:检测癌基因D52家族新基因PC-1在细胞周期各时相的表达变化。方法:用1mmol/L羟基脲处理前列腺癌细胞系LNCaP和C4-2B40h,使细胞同步化于G1/S期,在药物撤除后0～16h不同时间点收获细胞,分别进行流式分析和Western blot检测。结果:流式分析和Western blot检测在LNCaP和C4-2B细胞系中得到了趋势一致的结果,即PC-1基因在G2/M期高表达。结论:PC-1基因的表达与前列腺癌细胞的细胞周期有关,表明PC-1蛋白可能在G2/M期发挥作用。Objective： To investigate the expression of PC-1, the new gene of tumor protein D52 family, during cell cycle. Methods： Two prostate carcinoma cell lines LNCaP and C4-2B were treated by 1 mmol/L hydroxyurea for 40 h and synchronized to G1/S phase. Cells were harvested at different time points from 0 to 16 hours after hydroxyurea removing for flow cytometry analysis and Western blot. Results： Results of flow eytometry analysis and Western blot showed that in both LNCaP and C4-2B cell lines the expression of PC-1 gene was increased in G2/M phase. Conclusion： The expression of PC-1 gene is related with G2/M phase in cell cycle, suggesting that PC-1 may play a role in G2/M phase.

关 键 词：癌基因D52家族 PC-1基因 细胞周期 G2/M期 

分 类 号：Q279[生物学—细胞生物学] Q786