正常大鼠肝射频消融对脾树突状细胞的影响  

Influence of radiofrequency ablation on spleen dendritic cells in normal rats

作　　者：戴维德[1] 范智慧[1] 陈敏华[1] 河福金[2] 李洪民[2] 

机构地区：[1]北京大学临床肿瘤学院、北京肿瘤医院暨北京市肿瘤防治研究所超声科、恶性肿瘤发病机制及转化研究教育部重点实验室,100142 [2]北京大学临床肿瘤学院、北京肿瘤医院暨北京市肿瘤防治研究所病理科、恶性肿瘤发病机制及转化研究教育部重点实验室,100142

出　　处：《中华超声影像学杂志》2009年第7期625-627,共3页Chinese Journal of Ultrasonography

基　　金：北京市科委重大项目培育专项（Z0005190040431）

摘　　要：目的探讨正常大鼠肝射频消融（radio—frequency ablation，RFA）前后脾组织中单个核细胞表达OX62、OX6及CD86的变化和意义。方法18只健康SD大鼠均分为RFA1周组（6只）、RFA2周组（6只）和对照组（6只），分别在术前、术后1周、术后2周处死动物取出脾组织，采用流式细胞术检测大鼠脾单个核细胞OX62、OX6及CD86表达。同时做病理形态学观察。结果鼠肝组织射频灶病理组织学检查显示凝固性坏死的特点。正常大鼠的脾单个核细胞中（10．36±3．21）％表达大鼠特异性标志OX－62，RFA后1周为（18．03±5．70）％，RFA后2周为（12．63±8．0）％，RFA后1周组与正常大鼠组比较差异有统计学意义。0X－6在正常大鼠脾单个核细胞中的阳性表达率为（76．33±7．86）％，RFA后1周为（78．33±7．25）％，RFA后2周为（86．04±7．25）％，RFA后2周组与正常大鼠组比较差异有统计学意义。CD86在正常大鼠脾单个核细胞中的阳性表达率为（63．06±8．77）％，RFA后1周为（55．74±14．49）％，RFA后2周为（63．49±11．81）％。RFA后1周组及RFA后2周组与正常大鼠组比较差异均无统计学意义。结论RFA促使正常大鼠外周血中树突状细胞前体细胞迁移到脾组织中的数量增多，并进一步分化成熟，对提高机体在免疫应答中的抗原提呈能力起到促进作用。Objective To study the change of spleen Dendritic cells in normal rats treated by radio- frequency ahlation（RFA）. Methods Eighteen healthy SD rats were separated into group 1 week after RFA with 6 rats,group 2 week after RFA with 6 rats and control group with 6 rats. Spleen tissue were taken out respectively before RFA, 1 week after RFA and 2 weeks after RFA. The number and the phenotype of Dendritic cells in spleen were analyzed with flowcytometry. Results Pathologyical examination after RFA showed the characteristic that coagulation necrosis and cellular degeneration and granulation tissue forming appeared from target center to peripheral of the target. （10.36 ± 3.21）% of normal rat mononuclear cells in spleen express OX-62,the ratio became （18.03±5.7）% 1 week after RFA and （12. 63 ± 8. 0） % 2 weeks after RFA,the difference between group 1 week after RFA and control group was marked. （76.33 ± 7.86） of normal rat mononuclear cells in spleen express OX-6, the ratio became （78.33 ± 7.25）% 1 week after RFA and （86.04 ± 7.25） % 2 weeks after RFA, the difference between group 2 weeks after RFA and control group was marked. （63.06 ± 8.77） % of normal rat mononuclear cells in spleen express CD86,the ratio was （55.74± 14.49）% 1 week after RFA and （63.49 ± 11.81 ） % 2 weeks after RFA, the difference between groups 1 week or 2 weeks after RFA and control group was not marked. Conclusions RFA can increasing the number of precursor Dendritic cells migrating from peripheral blood to spleen, and those cells may furtherly differentiate or maturate, which may he contributed to improve the ability delivery of body to antigen to a certain extent.

关 键 词：导管消融术 肝 脾 树突细胞 

分 类 号：R686[医药卫生—骨科学]