舌根隧道法射频损伤的三维重建研究  

Channel radiofrequency volumetric reduction of tongue base a vitro porcine three-dimensional reconstruction experiment for the treatment of obstructive sleep apnea hypopnea syndrome

作　　者：关建[1] 易红良[1] 于栋祯[1] 黄艳艳[1] 殷善开[1] 

机构地区：[1]上海交通大学附属第六人民医院耳鼻咽喉科鼾症诊治中心,上海200233

出　　处：《临床耳鼻咽喉头颈外科杂志》2009年第15期700-702,705,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:研究舌根隧道法射频治疗中舌根不同部位损伤体积,探讨其治疗的安全性。方法:18个新鲜离体猪舌随机分成6个组,每组3只。参照Powell等介绍方法在猪舌根选取6个射频操作点。用低温等离子射频发生仪进行猪舌根射频操作,取能级6,作用时间10s。作用后的舌根组织块进行连续冷冻切片,间隔200μm保留切片1张,苏木精-伊红染色后,进行序列组织切片的全貌二维图像采集,对损伤区域进行图像分割,提取。将提取分割图像导入IPS图像处理软件,进行损伤区域的三维重建及体积计算。结果:各作用点体积分别为(359.5±5.6)mm3、(364.3±7.0)mm3、(363.7±7.2)mm3、(354.1±11.8)mm3、(349.4±17.2)mm3、(353.5±7.9)mm3,舌根各作用点射频损伤体积差异无统计学意义(P>0.05)。结论:在舌根隧道法射频治疗中,相同作用能级和作用时间下不同作用点损伤体积一致。低温等离子射频治疗系统作用能量集中,舌根区域损伤范围局限,在舌根区域操作具有较高的安全性。Objective：To investigate lesion size caused by channel radiofrequency volumetric reduction of porcine tongue base in vitro using the technique of three dimensional reconstruction. And to evaluate safety about channel radiofrequency volumetric reduction of tongue base. Method： Eighteen fresh porcine tongues were randomly separated into six groups,and each group had three ones. The tongue bases were designed six points according to description of Powell. Tongues base were acted on 10 s and 6 level by Coblation radiofrequency system and were cut into serial freezing histological sections. These segments were sectioned at 20 μm on the injury lesion and stained with H ＆ E. Collected 2D digital imagine of order histological sections, drawn and cut apart part of the lesion of these sections, Images were procesed IPS and were taken three-dimensional reconstruction and statistics analyzes with SPSS10. 0. Result： The mean value of tongue base lesion volumes among points was （359. 5± 5.6）mm^3,（364.3±7.0）mm^3,（363.7±7.2）mm^3,（354. 1±11.8）mm^3,（349.4±17.2）mm^3,（353.5±7.9）mm^3 separately. Statistic analysis hy one-way ANOVA showed that there was a insignificant difference between the groups（P〉0.05）. Conclusion：These results demonstrated no significant effect lesion size in channel radiofrequency volumetric reduction in the different points of the tongue base. These data also indicated that coblation radio/requency system is a safe method for obstructive sleep apnea hypopnea syndrome.

关 键 词：阻塞性睡眠呼吸暂停低通气综合征 射频 三维重建 舌根 

分 类 号：R563.8[医药卫生—呼吸系统]