Comparison Study of Chitosan/β-Cyclodextrin and Carboxymethyl Chitosan/β-Cyclodextrin Microspheres  

作　　者：LI Ruobao ZHAO Chunling ZHANG Weifen SHI Lihong TANG Jinbao 

机构地区：[1]Department of Basic Medicine, Weifang Medical University,Weifang 261053,Shandong, China [2]Shandong Provincial Key Laboratory of Applied Pharmacology, Weifang Medical University, Weifang 261053, Shandong, China

出　　处：《Wuhan University Journal of Natural Sciences》2009年第4期362-368,共7页武汉大学学报（自然科学英文版）

基　　金：Supported by the Natural Science Foundation of Shandong Province (Y2007C044 and Y2008F15);Scientific and Technological Brainstorm Project of Shandong Province (2008GG10002022)

摘　　要：To compare two microspheres of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin loaded theophylline as pulmonary sustained drug delivery carriers, the characteristics and ciliotoxicity were studied. The drug loadings of chitosan/ β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin micro- spheres were 21.09% and 21.42%, and the encapsulation efficiencies were 91.40% and 92.80%. The distributions of 50% （d0.5） of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin microspheres were 4.89 and 5.83 μm, respectively. Both micro- spheres showed spherical shape with smooth or wrinkled surfaces. FT-IR of chitosan/β-cyclodextrin microspheres demonstrated that theophylline had formed hydrogen bonds with chitosan and β-cyclodextrin, while for the carboxymethyl chitosan/β-cyclodextrin microspheres theophylline had interaction with carboxymethyl chitosan. The moisture absorption showed that an equilibrium was reached within 24 h. The two microspheres possessed better adaptability. In vitro release of theophylline from chitosan/ β-cyclodextrin microspheres was slower than that from carboxy- methyl chitosan/β-cyclodcxtrin microspheres at pH 6.8.To compare two microspheres of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin loaded theophylline as pulmonary sustained drug delivery carriers, the characteristics and ciliotoxicity were studied. The drug loadings of chitosan/ β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin micro- spheres were 21.09% and 21.42%, and the encapsulation efficiencies were 91.40% and 92.80%. The distributions of 50% （d0.5） of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin microspheres were 4.89 and 5.83 μm, respectively. Both micro- spheres showed spherical shape with smooth or wrinkled surfaces. FT-IR of chitosan/β-cyclodextrin microspheres demonstrated that theophylline had formed hydrogen bonds with chitosan and β-cyclodextrin, while for the carboxymethyl chitosan/β-cyclodextrin microspheres theophylline had interaction with carboxymethyl chitosan. The moisture absorption showed that an equilibrium was reached within 24 h. The two microspheres possessed better adaptability. In vitro release of theophylline from chitosan/ β-cyclodextrin microspheres was slower than that from carboxy- methyl chitosan/β-cyclodcxtrin microspheres at pH 6.8.

关 键 词：THEOPHYLLINE CHITOSAN carboxymethyl chitosan MICROSPHERES in vitro release 

分 类 号：O636.1[理学—高分子化学] O636.12[理学—化学]