行车人员β2-肾上腺素能受体基因多态性及与职业精神紧张的交互作用对原发性高血压发生的影响  

Roles of the interaction between β2-AR polymorphism and occupational mental stress in EH

作　　者：刘宝英[1] 周权[1] 钟萍[1] 杨华[1] 陈晋 郭志伟 

机构地区：[1]福建医科大学公共卫生学院,福州350004 [2]福州铁路卫生监督所

出　　处：《卫生研究》2009年第4期445-448,共4页Journal of Hygiene Research

基　　金：福建省医学创新课题(No.2007-CX-8)

摘　　要：目的探讨β2肾上腺素能受体(β2-AR)Arg16Gly基因多态性及与职业精神紧张的交互作用对原发性高血压(EH)发生的影响。方法以职业紧张高暴露的男性行车人群为基础的1∶1病例对照研究(95对),调查职业紧张、紧张反应、焦虑、A型行为等职业精神紧张及高血压的常见危险因素,应用PCR-RFLP技术检测β2-ARArg16Gly基因多态性。采用似然比检验判断因素效应之间是否有相乘模型OR交互=OR11/(OR01×OR10)的交互作用。结果病例组和对照组的β2-AR16位点基因Arg/Arg、Arg/Gly、Gly/Gly频率分布有差异,具有统计学意义(P<0.05),在调整BMI等影响因素后,携带Gly/Gly的个体患EH的OR值为2.759(95%CI:1.200～6.347);携带突变型Arg16Gly基因与紧张反应、特质焦虑对原发性高血压影响的交互作用符合相乘模型,当携带突变型Arg16Gly基因与高紧张反应、特质焦虑同时存在时,其患EH的风险分别增加至12.797(95%CI:3.379～48.461),40.862(95%CI:1.403～119.057)。结论β2-AR基因Arg16Gly多态性很可能是职业紧张高暴露男性行车人群EH发生的重要易感因素;携带突变型Arg16Gly基因的个体,暴露于紧张反应和特质焦虑时,其原发性高血压发生的危险性显著增加。Objectlve To explore the role of the interaction between β2-adrenergic receptor（β2-AR）gene Arg16Gly polymorphism and occupational mental stress in EH. Methods Using 1 ： 1 case-control study, we select 95 couple people as the study objects from the high-exposure occupational stress males who work on the train. Measuring occupational stress, stress reaction, anxiety, type A behavior and other occupational mental stress by using proper questionnaire, and applying PCR-RFLP technology to detect adrenergic receptor（β2-AR） Arg16Gly Polymorphism. Using likelihood ratio test to judge whether it exist the interaction between the potencies and fits the multiplicative model： OR = OR11/（ OR01 × OR10 ）. Results The distribution of Arg/Arg, Arg/Gly, Gly/Gly in essential hypertension cases and controls are significant difference （ P 〈 0.05 ）, Gly/Gly had a increased risk of hypertension 2.759 （95 % CI： 1. 200 - 6. 347） compared to Arg/Arg upon controling BMI and other factors. The interaction among Arg16Gly（A/G ＋ G/G） gene,occupational stress reaction and anxiety is fit the muhiplicative model. The results showed that when Arg16Gly（ A/G ＋ G/G） genotype interact with strain, the risk of EH will add to 12. 797（95 % CI：3. 379 - 48.461 ）. When it interact with anxiety, the risk of EH will add to 40. 862（95 % CI：1.403 - 119.057）. Conclusion Arg16Gly polymorphism of β2-AR might be the crucial risk factor for EH among high-exposure occupational stress males. Individual who has genotype Gly/Gly, while exposure of strain and T- Anxiety is much easier to get EH.

关 键 词：原发性高血压 Β2肾上腺素能受体 单核苷酸多态性 职业精神紧张 基因-环境交互作用 

分 类 号：R135.99[医药卫生—劳动卫生] R544.1[医药卫生—公共卫生与预防医学]