迟熟蕉柑茎尖嫁接及其病毒鉴定  

Shoot-tip Grafting of Late-ripening Tangan(Citrus tankan Tanaka) and Identification of Virus Diseases

作　　者：王明召 区善汉 娄兵海 刘升球 梅正敏 陈贵峰 麦适秋 陈宜超 莫健生 

机构地区：[1]广西柑桔研究所,广西桂林541004

出　　处：《南方园艺》2009年第4期3-4,8,共3页Southern Horticulture

基　　金：广西科技攻关项目(桂科攻0424002-3);广西农业重点科技计划项目(NK200829)

摘　　要：采用田间结果树上的芽作为接穗进行茎尖嫁接,最终获得7株茎尖嫁接苗。对所获得的茎尖嫁接苗,应用指示植物腊斯克枳橙、墨西哥来檬和伊特洛格香橼亚利桑那861-S-1分别鉴定柑橘碎叶病、衰退病和裂皮病,用PCR检测技术鉴定柑桔黄龙病。结果表明:1、3、4、5、6号迟熟蕉柑单株不带碎叶病、衰退病、裂皮病和黄龙病,可用作繁殖无病毒苗木的备选材料。Buds from grown trees in field were used as cions of shoot-tip grafting. ,and 7 seedlings were obtained by the shoot-tip grafting technique. Rusk citrange, Mexican lime and Citron 861-S-1 were used as indicator plants to identify Citrus Tatter Leaf Virus （CTLV）, Citrus Tristeza Virus （CTV） and Citrus Exocortis Viroid （CEVd）, respectively, and PCR technique was used to detecting Citrus Huanglongbing （HLB） on the 7 seedlings. The results were following, no pathogen of CTLV, CTV, CEVd and HLB could be fund from number 1, 3, 4, 5, 6 seedlings of Late-ripening Jiaogan , and can be used for virus-free seedling propa- gation .

关 键 词：迟熟蕉柑 茎尖嫁接 病毒鉴定 

分 类 号：S888.32[农业科学—特种经济动物饲养] S855.3[农业科学—畜牧兽医]