生发泡移植过程对小鼠卵母细胞体外受精的影响  

Effect of germinal vesicle translation process on in vitro fertilization of mouse oocytes

作　　者：崔龙波[1] 周雪莹[1] 崔志峰[2] 黄秀英[3] 孙方臻[3] 

机构地区：[1]烟台大学生物化学系,山东烟台264005 [2]烟台大学海洋学院,山东烟台264005 [3]中国科学院遗传与发育生物学研究所,北京100080

出　　处：《河北农业大学学报》2009年第4期96-99,共4页Journal of Hebei Agricultural University

基　　金：国家973项目(G1999055902);山东省自然科学基金课题(Y2008D30)资助

摘　　要：为研究生发泡(GV)移植后的重组卵母细胞能充分在体外成熟和发育,本试验比较了GV移植过程及体外成熟对小鼠卵母细胞体外受精的影响。通过透明带切口及GV移植获得的重组GV期卵母细胞的成熟率为87.0%(147/169),体外受精后发育到原核期胚和2-细胞期胚的比率分别为76.2%(112/147)和42.2%(62/147),与仅做透明带切口但不进行GV移植的对照组相似(P>0.05),表明GV移植过程未对卵母细胞体外受精后的早期胚胎发育能力产生不利影响。但与体内成熟的卵母细胞相比,体外成熟卵母细胞的受精率和卵裂率都明显降低,表明其进一步发育的能力受到影响。To study oocytes reconstructed by germinal velop fully in vitro, effects of GV translation process vesicle （GV） translation can mature and deand in vitro maturation on in vitro fertilization （IVM） of mouse oocytes were compared. The maturation rate of the reconstructed oocytes obtained by zona cutting and GV translation was 87.0% （147/169）, and the rates of pronuclear and 2-cell embryo developed from these oocytes after IVF were 76.2% （112/147）and 42.2% （62/ 147）, respectively, which were comparable （P 〉0.05） with the control oocytes obtained by zona cutting but not GV translation. The results showed the GV translation process had no harmful effect on early embryo developmental capacity of the oocytes after IVM. The rates of fertilization and cleavage of the oocytes matured in vitro, however, were significantly lower than that matured in vivo, showing the further developping capacity of the oocytes matured in vitro was affected.

关 键 词：卵母细胞 GV移植 体外成熟 体外受精 

分 类 号：Q419[生物学—生理学]