杀蚊苏云金芽孢杆菌Cry4Aa和Cry4Ba晶体蛋白结构的计算机对比分析  

Comparison and Analysis of Cry4Aa and Cry4Ba Proteins of Bacillus Thuringiensis with Computer

作　　者：吕媛[1] 夏立秋[1] 张允雷[1] 沈琳[1] 丁学知[1] 赵新民[1] 李文萍[1] 

机构地区：[1]湖南师范大学生命科学院和医学院、微生物分子生物学湖南省重点实验室,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2009年第2期81-84,共4页Journal of Natural Science of Hunan Normal University

基　　金：国家自然基金资助项目(30670052);国家863计划基金资助项目(2006AA02Z187,2006AA10A212);湖南省教育厅资助项目(08A036);湖南省教育厅大学生创新性实验计划(043-0094)

摘　　要：运用生物信息学软件对苏云金芽孢杆菌毒素Cry4Aa和Cry4Ba的基本参数、一级结构、二级结构、三级结构和跨膜区进行了预测比较.它们在一级结构上有较大差异,但二级结构和跨膜区相似,三级结构中各毒素的结构域Ⅱ、ⅡI之间基本相似,结构域I之间差异较大.Cry4蛋白之间的结构相似性和差异性与其作用机理和杀虫特异性有关.为分析杀蚊蛋白作用的分子机制、定点突变及研究高效的Bt生物杀虫剂有效控制蚊虫提供参考.The primary parameters, secondary and tertiary structures and transmembrane regions of Cry4Aa and Cry4Ba proteins of Bacillus and predicted using the software of bioinformatics, onsiderable differences are found among the two proteins in primary structure, and many similarities are in secondary structure and transmembrane regions. The structural diversity is smallest in domain II and domain III, and domain I is the most divergent domain. Those structural diversity and similarity may be involved in determining the action mode and insect specificity. That is greatly helpful to better understand the molecular mechanism of mosquito-killing protein andsite-directed mutagenesis, and provided reference for attaining high-performance biological insecti- cide Bt which could control mosquitoes efficiently.

关 键 词：苏云金芽孢杆菌 Cry4蛋白 序列 结构 对比分析 

分 类 号：O641[理学—物理化学]